二价锰和三价锰对神经瘤细胞线粒体膜电位的影响  

Effects of Manganese with Different Valences on Mitochondrial Membrane Potential in Human SH-SY5Y Cells in Vitro

作　　者：刘祯[1] 李国君[1] 张杰[1] 周春艺[1] 李鹏高[1] 赵春礼 杨慧 Wei Zheng 景鹏[4] 

机构地区：[1]首都医科大学公共卫生与家庭医学学院 [2]北京神经科学研究所 [3]School of Public Health,Columbia University [4]首都医科大学解剖研究室

出　　处：《首都医科大学学报》2002年第1期14-16,共3页Journal of Capital Medical University

基　　金：国家自然科学基金 ( 30 0 0 0 14 0 );教育部科学技术研究重点项目 ( 0 0 132 );北京市科技新星计划 ( 95 5 810 2 80 0 );教育部"高等学校骨干教师资助计划"项目 ( 5 2 );北京市教委基金资助课题

摘　　要：采用 0 .2 5、0 .5 0、0 .75mmol/L的二价锰和三价锰分别对神经瘤细胞 (SH SY5Y)染毒 2 4h ,通过线粒体特异性染料罗丹明 1 2 3进行荧光标记 ,利用激光扫描共聚焦显微镜观察染毒细胞的膜电位变化 ,并与未染毒的细胞对照组进行比较。结果表明二价锰和三价锰均可引起线粒体膜电位的改变 (P <0 .0 5 ) ,且随剂量增高 ,有降低趋势。此外 ,在 0 .5 0mmol/L和 0 .75mmol/L剂量下 ,三价锰引起的线粒体膜电位改变比二价锰更为明显。提示线粒体膜电位的改变是锰神经细胞毒性作用的重要环节。Human dopaminergic SH-SY5Y cells were exposed to manganese of bivalence and trivalence at the concentrations of 0.25, 0.50, 0.75 mmol/L for 24 h respectively. The mitochondrial potential was determined by incubating the culture with Rhodamine 123, a mitochondrial specific dye, followed by laser scanning confocal microtechnique (LCSM) analyses of fluorescence signal. Results showed that Mn(Ⅱ)and Mn(Ⅲ) treatment significantly decreased fluorescence signal as compared to the controls, suggesting a diminished mitochondrial membrane potential after Mn treatment. The effects were Mn-concentration dependent. In addition, we found that Mn(Ⅲ) appeared to be more cytotoxic than Mn(Ⅱ) in altering mitochondrial membrane potential at the concentration of 0.50 mmol/L and 0.75 mmol/L. These data confirm that mitochondria may serve as the primary target for Mn cytotoxicity by altering the membrane potential.

关 键 词：锰 激光扫描共聚焦显微镜 线粒体 膜电位 神经瘤细胞 二价锰 三价锰 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R338.8[医药卫生—临床医学]