bcl-2/bax基因在铁诱导肝细胞凋亡中的作用  

THE FUNCTION OF bcl-2/bax GENE IN HEPATOCYTES APOPTOSIS INDUCED BY IRON

作　　者：曹治宸[1] 白玉[1] 宋艳改[1] 甄真[1] 勾凌燕[2] 庄甲举[2] 刘景冬[2] 

机构地区：[1]河北医科大学第三医院感染科,石家庄050051 [2]白求恩军医学院科研中心

出　　处：《河北医科大学学报》2002年第2期77-80,共4页Journal of Hebei Medical University

摘　　要：目的研究bcl 2、bax基因在铁负荷诱导肝细胞凋亡中的作用。方法右旋糖酐铁复制大鼠高铁负荷模型 ,以CCl4攻击。观察各实验组 (高铁组、CCl4组、CCl4+高铁组 )和空白对照组的血清铁 (serumiron ,SI)、肝组织铁及丙二醛 (malondialdehyde ,MDA)含量变化 ,流式细胞仪检测肝细胞DNA含量 ,bcl 2、bax表达量 ,计算凋亡指数(apoptoticindex ,AI)和增殖指数 (poliferationindex ,PI)。 结果高铁组不仅大鼠SI、肝组织铁及MDA含量增加 ,而且AI升高 ,bax水平上调 ,PI无明显变化。CCl4+高铁组与高铁组相比 ,MDA、AI、bax均有显著增加。结论高铁负荷诱导肝细胞凋亡的机制可能为 :上调促凋亡基因bax水平 ,催化脂质过氧化反应 ,促进氧自由基产生。ObjectiveTo study the action of bcl 2/bax in the hepatocytes apoptosi induced by iron overload.MethodsRats models were made with iron dextran (ID) through intraperitoneal injection or with CCl 4 through intracutaneous injection, or with both of them. Then serum iron (SI), hepatic iron concentration (HIC) and malondialdehyde (MDA) levels were observed in experimental groups (iron overload, CCl 4 and CCl 4+ iron overload groups) and blank group. The hepatocytes apoptotic index (AI), proliferation index (PI) and bcl 2/bax expression were detected with flow cytometrie methed. ResultsThe increased SI, HIC and MDA levels, higher AI and bax expression could be found in ID injection group,while no significant change in PI was seen in the group. In addition, MDA, AI and bax were elevated more significantly in CCl 4 and ID injection group than in single ID injection group.ConclusionIt was found that iron overload may induce hepatocyte apoptosis by promoting bax expression and catalyzing lipid peroxidation reaction to produce free radial. Moreover, iron overload and CCl 4 have coordinated function to hepatocyte apoptosis.

关 键 词：铁右旋糖酐复合物 肝 细胞学 细胞死亡 bcl-2/bax基因 肝细胞凋亡 铁 肝损伤 

分 类 号：R361.3[医药卫生—病理学] R575[医药卫生—基础医学]