中药“口康”含漱液体外抑菌试验和毒性试验研究  

Antibacterial activity of Chinese medicine kou-Kang mouthwash and its acute toxicity test

作　　者：秦群[1] 谢兆霞[1] 皮海珍[1] 李新中[2] 谭达人[1] 

机构地区：[1]中南大学湘雅医院血液科,长沙410008 [2]中南大学湘雅医院药剂科,长沙410008

出　　处：《湖南医科大学学报》2002年第1期69-70,共2页Bulletin of Hunan Medical University

基　　金：省中医药管理局资助项目 [编号 983 2 9]

摘　　要：目的 :研究中药“口康”含漱液的抑菌作用及毒性 ,为临床治疗口腔感染疾病提供实验依据。方法 :用琼脂稀释法测定“口康”含漱液对 4 9株口腔致病菌的最低抑菌浓度 ,并进行了急性毒性试验。结果 :4 9株口腔致病菌中 ,2 7株革兰阴性杆菌最低抑菌浓度 (MinimalinhibitoryconcentrationMIC)范围 0 .0 3～ 1.0g·ml-1,抑制 5 0 %被试菌株的MIC(MIC50 )均为 0 .12 5g·ml-1,抑制 90 %被试菌株的MIC(MIC90 )为 0 .12 5～ 0 .5g·ml-1;2 1株革兰氏阳性球菌MIC范围0 .0 0 8～ 0 .5g·ml-1,MIC50 为 0 .0 15～ 0 .12 5g·ml-1,MIC90 为 0 .0 15～ 0 .5g·ml-1;1株白色念珠菌MIC为 0 .0 15g·ml-1。急性毒性试验显示 :最大耐受量为生药 4 17.6g·kg-1,达到临床日剂量 2 10倍 ,未见动物死亡和明显毒性反应。结论 :“口康”含漱液对各类不同口腔致病菌株均有抑菌作用 ;对革兰阳性球菌抑菌作用更佳 ,为革兰阴性杆菌的 2～ 8个对比倍数 ;对念珠性霉菌抑菌作用较强。该制剂毒性较小 。Objective To study the antibacterial activity of Chinese medicine kou Kang mouthwash and its acute toxicity, and provide the basis of experiment and therapy for infectious diseases of the oral cavity. Methods Minimal inhibitory concentrations (MIC) of kou Kang mouthwash were determined by the agar dilution method, and the acute toxicity was done. Results The range of MIC kou Kang mouthwash against 27 Gram negative bacterial were 0.03～1.0 g·ml -1 . MIC 50 was 0.125 g·ml -1 and MIC 90 was 0.125～0.5 g·ml -1 . The range of MIC kou Kang mouthwash against 21 Gram positive bacterial was 0.008～0.5 g·ml -1 . The range of MIC 50 and MIC 90 were 0.015～0.125 g·ml -1 and 0.015～0.5 g·ml -1 , respectively. The value of MIC was 0.015 g·ml -1 against candidia albicans. The acute toxic test indicated that the maximum tolerance dose was 417.6 g·kg -1 and 210 times as much as the clinical dose a day. No death of animals or toxicity and side effect occurred in the test. Conclusion Kou Kang mouthwash shows a potent antibacterial activity against various pathogens of the oral cavity. Its antibiotic activity against Gram positive bacteria was 2～8 times stronger than that against Gram negative bacteria. It has low toxicity and can be used in clinical application.

关 键 词：口腔疾病 细菌感染 口康含漱液 评价研究 动物实验 中药 毒性 体外抑菌 

分 类 号：R733.7[医药卫生—肿瘤]