大鼠脑缺血边缘区代谢物^1H—MRS的研究  

1H-MRS STUDY OF THE METABOLITE ON THE BORDER ZONE OF ACUTE CEREBRAL ISCHEMIA

作　　者：易黎[1] 卢广[1] 刘买利[1] 张苏明[2] 

机构地区：[1]中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室,武汉430071 [2]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《波谱学杂志》2002年第1期1-7,共7页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金重点资助项目(39730170);国家自然科学基金资助项目(39770810);国家自然科学基金资助项目(3

摘　　要：目的 利用不同的局灶性脑缺血模型，评价急性缺血后磁共振质子波谱（１Ｈ－ＭＲＳ）测 定缺血后边缘区脑组织代谢和生物能量变化的时空规律．为判定急性缺血预后，进行有效的 溶栓治疗提供有价值的生物化学信息． 方法 健康Ｓｐｒａｇｕｅ－Ｄａｗｌｙ大鼠９只，雌雄不拘，随机分 为两组． Ａ组（４只），自体血栓栓塞１ｈ； Ｂ组（５只），线栓法栓塞１ｈ．分别于栓塞后３０、４０、５０、６０ ｍｉｎ进行１Ｈ－ＭＲＳ检查，相对含量分析兴趣区（ｒｅｇｉｏｕｓ ｏｆ ｉｎｔｅｒｅｓｔ，ＲＯＩｓ）氮－乙酰天门冬 氨酸（ＮＡＡ）、胆碱（Ｃｈｏ）和乳酸（Ｌａｃ）等代谢产物的变化．结果 以ＮＡＡ、Ｃｈｏ、Ｌａｃ与磷酸肌 酸和肌酸（ＰＣｒ＋Ｃｒ）的谱峰积分面积比值为判断标准，上述各代谢产物在兴趣区内于缺血后 １ｈ内逐渐下降．其中缺血后６０ｍｉｎ．Ｃｈｏ／（ＰＣｒ＋Ｃｒ）、ＮＡＡ／（ＰＣｒ＋Ｃｒ）及Ｌａｃ／（ＰＣｒ＋Ｃｒ）的比 值与缺血后５０ ｍｉｎ的比值统计学有显著性差异（Ｐ＜０．０５）结论 １Ｈ－ＭＲＳ技术为研究急性 缺血性卒中后脑细胞代谢、生化能量状态提供了一个无创性、直接性、综合性的研究工具．Objective The use of different models of focal cerebral ischemia to appraise the time-space rules of 1H-MRS on measuring metabolism and energy changes in the post-is- chemia brain tissue provides the clinical doctors with valuable information to judge the prog- nosis and carry out more effective therapy from the biochemical point of view. Methods 9 healthy Sprague-Dawly rats( indiscriminate the sex) was divided into two groups randomly. Group A (4 rats), occluded with self-thrombus for 1 h; Group B (5 rats), occluded with thread-emboli for 1 h. The 1H-MRS was examined in 30, 40, 50, 60 mm after occlusion re- spectively, and the metabolic changes of NAA, Cho and L.ac in the regions of interest in partly-fixed quantity were analyzed. Results Set the resonance intregrated area ratio of NAA, Cho, Lac to Pcr+Cr as the criterion, the values of all the metabolites declined gradu- ally within 1 h after ischemia. Especially, the ratio of Cho/ (Pcr + Cr)、 NAA/ ( Pcr + Cr) and Lac/(Pcr+ Cr)in 60 mm has significant difference from that in 50 mm (P<0.05). Conclu- sions .Magnetic Resonance Proton Spectroscopy is a good research tool, which is straight- forward, comprehensive and no damage, for the study of cellular metabolism and the status of the biochemical energy in acute ischemia stroke.

关 键 词：磁共振质子波谱 脑缺血 ^1H-MRS 缺血边缘区 代谢物 溶栓治疗 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R816.1[医药卫生—临床医学]