孤立性纤维性肿瘤中GRIA2和STAT6的表达及意义  

Expression of GRIA2 and STAT6 in solitary fibrous tumor and their significance

作　　者：曹钟[1] 魏建国[2] 岑红兵 秦灵芝 敖启林[3] CAO Zhong;WEI Jian-guo;CEN Hong-bing;QIN Ling-zhi;AO Qi-lin(Department of Pathology,Hubei Huanggang Central Hospital,Huanggang 438000,China;Department of Pathology,Zhejiang Shaoxing People's Hospital,Shaoxing 312000,China;Institute of Pathology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]湖北省黄冈市中心医院病理科,黄冈438000 [2]浙江省绍兴市人民医院病理科,绍兴312000 [3]华中科技大学同济医学院附属同济医院病理研究所/同济医学院病理学系,武汉430030

出　　处：《临床与实验病理学杂志》2018年第11期1194-1199,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金青年科学基金(81600015)

摘　　要：目的探讨孤立性纤维性肿瘤(solitary fibrous tumor,SFT)中离子型谷氨酸受体2 (ionotropic glutamate receptor 2,GRIA2)与信号转导和转录激活因子6(signal transducer and activators of transcription 6,STAT6)的表达及意义。方法回顾性分析48例SFT的临床病理及免疫表型特征,并复习相关文献。采用免疫组化En Vision法检测48例SFT及60例其他软组织肿瘤中GRIA2、STAT6、PAX8和CD34的表达。比较上述标志物在诊断和鉴别诊断SFT中的价值。结果 (1)在SFT中,GRIA2和STAT6的阳性率分别为91. 7%(44/48)和95. 8%(46/48),在对照组中两者阳性率分别为3. 3%(2/60)和11. 7%(7/60),两种标志物在两组之间差异有显著性(P均<0. 05)。GRIA2和STAT6在SFT中表达的特异度和敏感度分别为91. 7%(44/48)、96. 7%(58/60); 95. 8%(46/48)、88. 3%(53/60)。(2)在SFT中,CD34、PAX8、BCL-2和CD99的阳性率分别为81. 3%(39/48)、45. 8%(22/48)、66. 7%(32/48)和60. 4%(29/48),上述4种标志物分别与GRIA2和STAT6的表达差异均有统计学意义(P均<0. 05)。(3)在SFT中,GRIA2和STAT6的表达与患者年龄、性别、肿瘤大小、部位、复发、恶性与否无关(P均> 0. 05)。结论 GRIA2和STAT6在SFT中分别呈细胞质和细胞核阳性的表达模式,是SFT独特的免疫学特征,采用两种标志物可以作为强有力的辅助诊断工具。Purpose To investigate the clinicopathological features,diagnostic and differential diagnostic value of ionotropic glutamate receptor 2( GRIA2) and signal transducer and activators of transcription 6( STAT6) in solitary fibrous tumor( SFT). Methods The clinicopathological data of 48 cases of SFT were retrospectively analyzed,and related literatures were reviewed. Immunohistochemical method( En Vision) was imposed to detect the expression of GRIA2,STAT6,PAX8 and CD34 in 48 cases of SFT and 60 cases of other soft tissue tumors,compared with the value of above markers in diagnosis and differential diagnosis of SFT. Results The positive expression rates of GRIA2 and STAT6 in SFT were 91. 7%( 44/48)and 95. 8%( 46/48) respectively,that were 3. 3%( 2/60)and 11. 7%( 7/60) in control group respectively. There were significant statistical differences between the two groups in both markers( P < 0. 05). The specificity and sensitivity of GRIA2 in SFT were 91. 7%( 44/48) and 96. 7%( 58/60). The specificity and sensitivity of STAT6 were 95. 8% and 88. 3%( 53/60),respectively. The positive expression rates of CD34,PAX8,BCL-2 and CD99 in SFT were 81. 3%( 39/48),45. 8%( 22/48),and 66. 7%( 32/48) and 60. 4%( 29/48). The difference in expression of GRIA2 and STAT6 between above four markers was statistically significant( P < 0. 05). The positive expression rates of GRIA2 and STAT6 in SFT were not related to age,gender,tumor size,location,recurrence and malignancy( P > 0. 05). Conclusion The positive expression patterns of GRIA2 and STAT6 in cytoplasm and nucleus are unique immunological features of SFT,utility of both markers as robust adjunct diagnostic tool.

关 键 词：孤立性纤维性肿瘤 离子型谷氨酸受体2 信号转导和转录激活因子6 免疫组织化学 

分 类 号：R738.6[医药卫生—肿瘤]