游泳激活AMPK通路治疗高脂日粮引起的非酒精性脂肪肝  

Swimming Activates AMPK Signaling Pathway to Treat Nonalcoholic Fatty Liver Caused by Highfat Diet

作　　者：傅建[1] 齐洁[2] 张钧[2] FU Jian;QI Jie;ZHANG Jun(College Of Physical Education,Yangzhou University,YangZhou25127,China;College Of Physical Education,Shanghai Normal University,Shanghai200234,China)

机构地区：[1]扬州大学体育学院,扬州225009 [2]上海师范大学体育学院,上海200234

出　　处：《体育与科学》2018年第6期84-91,113,共9页Sports & Science

基　　金：国家自然科学基金;项目号:31701037

摘　　要：背景与目的:非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)的流行与加剧给人类健康带来了巨大威胁。尽管运动对NAFLD具有疗效,但有关不同运动方案(运动类型、时长与强度)对NAFLD的治疗效果以及相关机制的研究还很欠缺。本研究旨在探讨不同强度的游泳运动对高脂饮食所诱导的大鼠NAFLD的治疗作用及相关机制。材料与方法:以6周龄Sprague-Dawley(SD)纯系雄性大鼠为研究对象,随机分为4组:普通饲料对照组(C组)、高脂饮食NAFLD组(H组)、高脂饮食+60min运动治疗组(HE60组)、高脂饮食+90min运动治疗组(HE90组),持续喂养至20周。通过测定体重、肝重、肝功指标、血脂、肝脂和组织切片评价运动对NAFLD相关性状的影响,通过免疫印迹和定量PCR测定关键信号通路蛋白质和mRNA表达水平。结果:60min和90min的游泳运动可以显著抑制高脂饮食引起的大鼠体重和肝重的增加,抑制高脂饮食引起的大鼠血脂、ALT和AST的升高,抑制高脂饮食引起的大鼠肝脏脂肪变性,并削弱高脂饮食对相关信号通路中关键分子(pAkt、AMPKα、PPARα、SREBP-1c、SCD1)的蛋白质和mRNA表达水平的影响。虽然60min和90min游泳运动疗效间差异不显著,但存在运动时间越长,疗效越好的趋势。结论:游泳运动对高脂日粮引起的大鼠NAFLD具有治疗作用,运动时间长和其他配套措施会增进。游泳运动产生疗效的机制包括:(1)提高肝脏中Akt的磷酸化水平,改善肝脏对胰岛素的敏感性;(2)增加肝脏中AMPKα的表达,抑制SREBP-1c和SCD1基因的表达和脂肪的合成;(3)增加肝脏中PPARα的表达来增强脂肪酸的氧化。本研究为NAFLD的运动治疗方案优化以及运动治疗机制的阐明提供了有用的信息。BACKGROUND&OBJECTIVE:The prevalence of non--alcoholic fatty liver disease(NAFLD)poses a great threat to human hearth.Although exercise is effective for NAFLD,there is still a lack of research on the effects of different exercise regimens (exercise types,duration and intensity)on NAFLD and the related mechanisms.The aim of this study was to investigate the therapeutic effect of swimming with different intensity on NAFLD induced by high fat diet in rats and its related mechanisms.MATERIALS AND METHODS:Six-week-old Sprague-Dawley(SD)male rats were randomly divided into four groups:Group C(fed with normal diet,control group),Group H(fed with high-fat diet),Group HE60(fed with high-fat diet,60minutes of swimming)and Group HE90(fed with high-fat diet,90minutes swimming).The effects of swimming exercise on NAFLD-related traits were evaluated by measuring body weight,liver weight,liver function index,blood lipids,liver lipids and tissue sections,and the protein and mRNA expression of some key molecules on the relevant signaling pathways were determined by Western blotting and quantitative PCR analyses.RESULTS:60min and90min swimming exercise could significantly inhibit the increase of body weight and liver weight induced by high fat diet,inhibit the elevation of blood lipids,ALT and AST,inhibit hepatic steatosis induced by high fat diet,and alleviate the effect of high fat diet on the protein and mRNA expression levels of key molecules(pAkt,AMPKα, PPARα,SREBP-1c,SCD1)on the relevant signaling pathways.Although there was no significant difference between60minutes and90minutes of swimming,it appeared that the longer the swimming time was,the better the effect was.CONCLUSIONS: Swimming exercise has therapeutic effect on high fat diet-induced NAFLD in rats,and long exercise time and other supporting measures can enhance the effect.The mechanisms underlying the therapeutic effect of swimming include:(1)increasing the phosphorylation level of Akt in the liver and improving insulin sensitivity of the liver;(2)increasing the e

关 键 词：非酒精性脂肪肝 游泳 高脂饮食 AMPKα PPARΑ AKT 

分 类 号：G804[文化科学—运动人体科学]