PTEN和hTERT在食管癌中的表达关系及意义  

Expressions and significances of PTEN and HTERT in esophageal cancer

作　　者：丁海滨[1] 杨勇[2] Ding Haibin;Yango Yong(Department of General Thoracic Surgery,the First People's Hospital of Tianmen City of Hubei Province,Tianmen 431700,China;Department of Oncology,the First People's Hospital of Tianmen City ofHubei Province,Tianmen 431700,China)

机构地区：[1]湖北省天门市第一人民医院普胸外科,431700 [2]湖北省天门市第一人民医院病理科,431700

出　　处：《中国医师杂志》2018年第11期1660-1664,1669,共6页Journal of Chinese Physician

摘　　要：目的探究磷酸酶及张力蛋白同源基因(PTEN)和人端粒酶反转录酶(h TERT)在食管癌中的表达关系及意义。方法收集本院于2010年12月至2012年12月期间行手术切除的84例食管癌标本为研究对象,同时取30例相应癌旁组织和30例正常食管黏膜组织作为对照。抗生蛋白链菌素-过氧化物酶(SP)免疫组化方法检测食管组织标本中PTEN和h TERT表达情况,并结合临床资料分析食管癌组织PTEN、h TERT与患者临床病理特征及预后的关系。结果⑴PTEN在食管癌组织中阳性表达率显著低于癌旁组织(χ2=5. 077,P=0. 024)和正常黏膜组织(χ2=22. 810,P=0. 000); PTEN在癌旁组织中阳性表达率显著低于正常黏膜组织(χ2=5. 104,P=0. 024)。⑵h TERT在食管癌组织中阳性表达率显著高于癌旁组织(χ2=19. 724,P=0. 000)和正常黏膜组织(χ2=46. 392,P=0. 000); h TERT在癌旁组织中阳性表达率显著高于正常黏膜组织(χ2=4. 565,P=0. 033)。⑶食管癌组织PTEN、h TERT表达强度与患者的肿瘤浸润程度、临床分期、淋巴结转移、分化程度有关(P <0. 05)。⑷至随访结束,全部患者的中位生存期为22个月,术后1、3、5年的累积生存率分别为65. 48%、38. 10%、28. 57%。PTEN低表达患者和PTEN高表达患者术后1、3、5年的累积生存率分别为60. 00%和84. 21%、30. 77%和63. 16%、23. 08%和47. 37%,PTEN高表达患者的生存率显著高于PTEN低表达患者(χ2=5. 073,P=0. 024)。h TERT低表达患者和h TERT高表达患者术后1、3、5年的累积生存率分别为76. 47%和58. 00%、50. 00%和30. 00%、38. 24%和22. 00%,h TERT高表达患者的生存率显著低于h TERT低表达患者(χ2=4. 174,P=0. 041)。结论在食管癌组织中PTEN呈低表达,h TERT呈高表达,均可作为食管癌患者临床预后的预测指标。Objective To investigate the expressions and significances of phosphatase and tension homologous genes (PTEN)and human telomerase reverse transcriptase (hTERT)in esophageal cancer. Methods 84 specimens of esophageal cancer underwent surgical resection from December 2010 to December 2012 were collected,at the same time,30cases of para cancerous tissues and 30 normal esophageal mucosa tissues were taken as control.Streptavidin peroxidase (SP)immune-histochemical method was used to detect the expressions of PTEN and hTERT in the esophageal tissue specimens,according to the clinical data,the relationships between PTEN,hTERT in the esophageal cancer tissues with clinical pathological features and prognosis was analyzed.Results (1)The positive expression rate of PTEN in esophageal cancer tissue was significantly lower than that in para cancerous tissue (χ^2=5.077,P =0.024)and normal mucosa tissue (χ^2=22.810,P =0.000);the positive expression rate of PTEN in para cancer tissues was significantly lower than that in normal mucosa (χ^2=5.104,P =0.024).(2)The positive expression rate of hTERT in esophageal cancer tissues was significantly higher than that in para cancerous tissue (χ^2= 19.724,P =0.000)and normal mucosa tissue (χ^2=46.392,P =0.000);The positive expression rate of hTERT in para cancerous tissues was significantly higher than that in normal mucosa (χ^2=4.565,P = 0.033).(3)The expression intensities of PTEN and hTERT in esophageal cancer tissues were related to the degree of tumor invasion,clinical stage,lymph node metastasis and degree of differentiation (P <0.05).(4)At the end of the follow-up,the median survival period of all patients was 22 months,the cumulative survival rates of 1,3,and 5 years after operation were 65.48%,38.10% and 28.57% respectively.The cumulative survival rates of 1,3,and 5 years after operation in patients with low expression of PTEN and patients with high expression of PTEN were 60.00% and 84.21%,30.77% and 63.16%,23.08% and 47.37%,respectively.The survival rate of patients wit

关 键 词：食管肿瘤/病理学 PTEN磷酸水解酶/代谢 端粒 末端转移酶/代谢 

分 类 号：R735.1[医药卫生—肿瘤]