Exo探针-RPA法现场快检牛肉产品中猪肉成分  

Detection of pork ingredient in beef product by exo probe-RPA method on-site rapid

作　　者：林霖[1] 王坤[1] 吴佳辉[1] 朱成杰 朱宇薇 张世伟[1] 赖心田[1] 杨国武[1] LIN Lin;WANG Kun;WU Jia-hui;ZHU Cheng-jie;ZHU Yu-wei;ZHANG Shi-wei;LAI Xin-tian;YANG Guo-wu(Shenzhen Academy of Metrology &Quality inspection,Shenzhen 518131;Shenzhen Mingde Experimental School,Shenzhen 518000)

机构地区：[1]深圳市计量质量检测研究院,深圳518131 [2]深圳明德实验学校,深圳518000

出　　处：《食品科技》2018年第11期349-353,共5页Food Science and Technology

基　　金：广东省质量技术监督局科技项目(2016PZ11)

摘　　要：目的:为方便监管,实现现场快速检测牛肉产品中猪肉成分。方法:通过在细胞色素C氧化酶亚型I中的猪特异性DNA序列上设计RPA引物以及exo探针,对引物对进行组合筛选,结合核酸快速提取方法,建立exo探针-RPA法快速检测方法。对其特异性、灵敏度测试,通过方法比对实验验证其检测准确性。结果:所建立的检测方法具有很好的特异性,低至1%(w/w)猪肉含量可被检出,与标准方法比对结果显示检测结果一致。结论:成功建立了牛肉产品中猪肉成分的exo探针-RPA现场快速检测法。Purpose: In order to realization on-site rapid detection the adulteration of pork in beef products and convenience government monitoring. Methods: Design RPA primer pairs and exo probe according to COI gene sequences specific for pig. Construct on-site rapid detection method through primer pairs screening, specificity and sensitivity test. Results: The method constructed has good specificity, and the sensitivity can be reach 1%(w/w). The testing results are the same with standard methods. Conclusions: This study successfully construct on-site rapid detection the adulteration of pork in beef products based on exo probe-RPA technology.

关 键 词：牛肉产品 猪DNA成分 重组聚合酶等温扩增技术 现场快检 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]