云南香料烟中1个新黄酮及其抗菌活性  

A new flavone from leaves of Nicotiana tabacum and its antibacterial activity

作　　者：吴玉萍[1,2] 李雪梅 孔维松 耿永勤 刘欣 杜刚 杨海英[3] 李晶[2] 胡秋芬 WU Yu-ping;LI Xue-mei;KONG Wei-song;GENG Yong-qin;LILT Xin;DU Gang;YANG Hai-ying;LI Jing;HU Qiu-fen(Yunnan Academy of Tobacco Agricultural Sciences,Yuxi 653100,China;Technology Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650106,China;Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission &Ministry of Education,Yumaan Minzu University,Kunming 650500,China)

机构地区：[1]云南省烟草农业科学研究院,云南玉溪653100 [2]云南中烟工业有限责任公司技术中心,云南昆明650106 [3]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500

出　　处：《中草药》2018年第22期5238-5241,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21462051);中国烟草总公司科技项目(110201502006);云南中烟科技项目(2016JC03)

摘　　要：目的对云南香料烟的化学成分进行研究,试图从中发现新活性化合物。方法运用硅胶、凝胶及RP-HPLC等多种色谱技术进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从云南香料烟中分离得到2个烷基取代的黄酮类化合物,分别鉴定为2′-羟基-7-(2-羟乙基)-8-甲氧基-黄酮(1)和烟草黄酮A(2)。对化合物1和2进行了抗菌活性筛选,其对耐甲氧西林金黄色葡萄球菌(MRSA)菌株的MIC90值分别为(38±4)和(33±3)μg/m L。结论化合物1为新化合物,命名为香料烟黄酮A。化合物2首次从香料烟中分离到,化合物1和2均具有显著的抗菌活性。Objective To fred the new biological compounds,the research of the chemical constituents in the leaves of Nicotiana tabacum had been carried out.Methods The chemical constituents in the leaves of N.tabacum were isolated by silica gel,Sephadex LH-20column chromatographies,and RP-HPLC methods.The structures of isolations were detected by using various spectroscopic techniques,including extensive 1D and 2D NMR techniques.Results Two C-alkylated fiavones were isolated from 95%ethanol extract of plant,which identified as 2'-hydroxy-7-(3-hydroxypropyl)-8-methoxy-fiavone (1)and tabaflavone A (2).Compounds 1and 2were evaluated for their anti-methicillin-resistant Staphylococcus aureus (anti-MRSA)activity.Conclusion Compound 2is isolated from the leaves of N.tabacum for the first time.Compound 1is a new compound named orientalflavone A.Compounds 1 and 2also show good anti-MRSA activity with MICg0value of (38±4)and (33±3)μg/mL,respectively.

关 键 词：香料烟 黄酮 香料烟黄酮A 烟草黄酮A 抗菌活性 

分 类 号：R284.1[医药卫生—中药学]