低强度脉冲超声对大鼠牙本质损伤修复过程中钙离子转运相关蛋白表达的影响  

The effects of low intensity pulsed ultrasound on the expression of calcium transportation associated proteins during dentin-pulp complex injury and repair in rats

作　　者：左静[1] 甄佳秀 杨正艳[1] 李月恒[1] 罗俊 ZUO Jing;ZHEN Jiaxiu;YANG Zhengyan;LI Yueheng;LUO Jun(Stomatological Hospital of Chongqing Medical University,Chongqing Key Laboratory of Oral Disease and Biomedical Sciences,China,401147;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education)

机构地区：[1]重庆医科大学附属口腔医院.口腔疾病与生物医学重庆市重点实验室,401147 [2]重庆市高校市级口腔生物医学工程重点实验室

出　　处：《实用口腔医学杂志》2018年第1期33-38,共6页Journal of Practical Stomatology

基　　金：重庆市卫生局重点科研项目(编号:2013-1-032);重庆高校创新团队建设计划(编号:XTDG201602006);重庆市科委基础与前沿研究计划(编号:cstc2016jcyjA0202)

摘　　要：目的:研究低强度脉冲超声(LIPUS)辐照SD大鼠牙本质损伤模型后对成牙本质细胞及牙髓细胞上钙离子转运相关蛋白的影响。方法:40只SD大鼠[(360±13)g]右侧上颌第一磨牙备洞建立牙本质损伤模型,随机对其中20只大鼠备洞牙及剩余20只大鼠左侧上颌第一磨牙予以超声辐照(强度30 mw/cm^2,频率1.5 MHz,频宽200μm,重复率1 kHz),形成备洞组、备洞+LIPUS组、LIPUS组及空白对照组(n=10),超声辐照每日1次,持续20 min,分别在辐照1、3、7、14 d后处死实验动物,应用HE染色观察牙本质-牙髓复合体组织结构及细胞形态变化,免疫组化染色及图像分析检测钙离子转运相关蛋白(Cav 1.2、NCX1)的表达差异。结果:组织形态学分析发现牙本质损伤后LIPUS可协同炎症反应促进第三期牙本质的形成,而LIPUS辐照正常牙未见明显病理改变出现。免疫组化分析发现备洞+LIPUS组在1 d及3 d时较备洞组各蛋白表达明显升高(P<0.05);LIPUS组1 d时Cav1.2表达较空白对照组高(P<0.05),其余时间点两者无差异。结论:LIPUS可能在牙本质损伤早期积极调控钙离子转运相关蛋白,加快第三期牙本质修复进程,其作用机制可能与炎症反应及机械效应相关。Objective:To investigate low intensity pulsed ultrasound(LIPUS)on the expression of L-type calcium ion channels (cavl.2)and Na^+-Ca^2+exchangers(NCX1)during dentin-pulp complex injury and repair in rats.Methods:Cavity preparation was made on the upper right first molar of40male adult SD rats,20of them and the upper left first molar of the other20were randomly chosen for LIPUS irradiation(frequency:1.5MHz,200μs pulses,pulse repetition frequency:1KHz,ISATA30mW/cm^2,20 min/d),so the animals were randomly allocated into4groups(n=10):Control group,LIPUS group,cavity preparation group and cavity preparation+LIPUS group.At1,3,7,14d post-irradiation the rats were sacrificed respectively for HE stain and immunohistochemical analysis.Results:Reparative dentin formation was observed at14days after cavity preparation and LIPUS irradiation,the expression of Cav1.2(L-type)and NCX1in this group were increased significantly at day 1 and day3.Compared with the control group, the expression of Car1.2in LIPUS group increased at day1post-irradiation.Conclusion:LIPUS may enhance tertiary dentin formation and up-regulate the expression of Carl.2and NCX1at the early period of dentin injury.

关 键 词：低强度脉冲超声 L型钙离子通道蛋白Cav1.2 钠钙交换体NCX1 牙本质牙髓复合体 

分 类 号：R781.3[医药卫生—口腔医学]