环介导等温扩增技术检测食品安全的研究进展  

Research Progress of Loop-Mediated Isothermal Amplification in Food Safety Testing

作　　者：王静[1] 许鑫[1] 王雪雨 姚伦广[1] 阚云超[1] 冀君[1] WANG Jing;XU Xin;WANG Xue-yu;YAO Lun-guang;KAN Yun-chao;JI Jun(Center for Nanyang Veterinary Biological Engineering Technology/Henan Provincial Engineering Laboratory of Insect Bio-reactor,Nanyang Normal University,Nanyang 473061,China)

机构地区：[1]南阳师范学院南阳市兽医生物工程技术研究中心/南阳师范学院昆虫生物反应器河南省工程实验室

出　　处：《中国生物工程杂志》2018年第11期84-91,共8页China Biotechnology

基　　金：河南省高等学校重点科研项目(18A230012);南阳师范学院青年项目(16134);南阳师范学院研究生创新基金(2017CX099)资助项目

摘　　要：食品安全一直是全世界公众健康的关注焦点。环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种特异性强、灵敏度高、快速简便的等温核酸扩增技术,近年来在核酸检测领域有着广泛的研究和应用。将LAMP技术应用到食品安全检测领域,可以快速准确的监控一些食品安全问题,避免对人类健康所构成的危害。因此针对LAMP技术在食品安全检测方面的优势进行分析,并结合LAMP技术与新兴诊断技术平台的联合运用进行了展望。Food safety has been the focus of public health throughout the world.Loop-mediated isothermal amplification (LAMP )technique is a kind of simple and rapid isothermal nucleic acid amplification technology with high specificity,and outstanding sensitivity.Recently,LAMP has been widely evaluated and applied in the field of nucleic-acid detection.LAMP technology can be applied to the food safety detection field,quickly and accurately monitored in a number of food safety problems harm to human health.Therefore,the advantages of LAMP technology in food safety detection are analyzed and combined with the combination of LAMP technology and new diagnostic technology platform.

关 键 词：环介导等温扩增 食品安全 核酸检测 联合应用 

分 类 号：Q52[生物学—生物化学]