组织蛋白酶H在甲状腺乳头状癌中的表达及其意义  

Expression of cathepsin H in papillary thyroid carcinoma and its significance

作　　者：周涛[1] 吕云霞[1] 陈万志[1] 许德斌[1] 谢嵘[1] 熊骋峰 张书勇 余济春[1] ZHOU Tao;LU Yunxia;CHEN Wanzhi;XU Debin;XIE Rong;XIONG Chengfeng;ZHANG Shuyong;YU Jichun(Department of Thyroid Surgery,the Second Affiliated Hospital,Nanchang University,Nanchang330006,China)

机构地区：[1]南昌大学第二附属医院甲状腺外科,江西南昌330006

出　　处：《中国普通外科杂志》2018年第11期1402-1408,共7页China Journal of General Surgery

基　　金：南昌大学研究生创新专项资金资助项目(cx2016332)

摘　　要：目的:探讨组织蛋白酶H(CTSH)在甲状腺乳头状癌(PTC)中的表达及其临床意义。方法:收集50例PTC及其癌旁组织和50例正常甲状腺组织,分别用qRT-PCR、Western blot及免疫组化检测CTSH的mRNA与蛋白的表达量,以及CTSH蛋白阳性表达率,并分析CTSH蛋白表达与患者临床病理因素的关系。结果:PTC组织中CTSH mRNA与蛋白的表达量,以及蛋白阳性表达率均明显高于癌旁组织和正常甲状腺组织(均P<0.05),而在癌旁组织与正常甲状腺组织中以上指标均无统计学差异(均P>0.05)。PTC患者中,有被膜浸润与淋巴结转移的患者CTSH蛋白阳性表达率明显高于无被膜浸润与无淋巴结转移者(均P<0.05)。结论:PTC组织中CTSH表达升高,且CTSH的高表达可能与PTC的恶性进展密切相关。Objective:To investigate the expression of cathepsin H(CTSH)in papillary thyroid carcinoma(PTC)and its clinical significance. Methods:The50paired specimens of PTC and adjacent thyroid tissue as well as50specimens of normal thyroid tissue were collected.The expressions of CTSH mRNA and protein as well as the positive expression rates of CTSH protein in these specimens were determined by qRT-PCR, Western blot and immunohistochemical staining,respectively. Then,the relations of CTSH protein expression with the clinicopathologic variables of PTC patients were analyzed. Results:Both CTSH mRNA and protein expression levels as well as the positive expression rate of CTSH protein in PTC tissue were significantly higher than those in tumor adjacent tissue and normal thyroid tissue(all P<0.05), while above indexes showed no significant differences between tumor adjacent tissue and normal thyroid tissue(all P>0.05).In PTC patients,the positive expression rate of CTSH protein in cases with capsular invasion or lymph node metastasis was significantly higher than in those without capsular invasion or lymph node metastasis(both P<0.05). Conclusion:The CTSH expression is increased in PTC tissue,and the increased CTSH expression may be closely associated with the malignant progression of PTC.

关 键 词：甲状腺肿瘤 癌 乳头状 组织蛋白酶H 肿瘤侵润 

分 类 号：R736.1[医药卫生—肿瘤]