机构地区:[1]南京农业大学资源与环境科学学院,南京210095 [2]中国农业科学院茶叶研究所,杭州310008 [3]云南省普洱茶树良种场,普洱665000
出 处:《生态学报》2018年第22期8158-8166,共9页Acta Ecologica Sinica
基 金:国家重点研发计划项目(2016YFD0200900);国家茶叶产业技术体系(CARS 19);中国农业科技创新工程项目(CAAS-ASTIP-2017-TRICAAS)
摘 要:为揭示不同施肥方式对茶园土壤真菌群落结构的影响,依托定位施肥试验,采集对照(CK)、纯化肥(N300)、有机肥配施(OM30) 3个处理的0—10 cm和10—20 cm两层土壤,通过高通量测序技术,分析不同施肥处理下,茶园土壤真菌群落结构特征及其与土壤理化性质的关系。结果表明0—10 cm土层中真菌Alpha多样性显著高于10—20 cm土层(P<0.05);在同一土层中,Alpha多样性变化趋势为CK> N300> OM30。子囊菌门、担子菌门、接合菌门是试验茶园土壤中三大优势真菌门类,Sordariomycetes纲,Tremellomycetes纲和Mortierellomycotina纲分别是子囊菌门、担子菌门和接合菌门下的优势种群。子囊菌门和担子菌门在0—10 cm土层中相对丰度较高,而接合菌门则在10—20 cm土层中具有较高丰度(P<0.05)。施肥处理提高了土壤中接合菌门的相对丰度,降低了子囊菌门的相对丰度。不同深度土壤中真菌群落结构差异主要受土壤理化性质变化驱动,冗余分析和Monte Carlo置换检验结果显示,土壤有机碳、全氮、碳氮比、全钾、速效钾含量对土壤真菌群落结构影响显著(P<0.05),而试验茶园土壤的pH变化对同一土层中真菌群落结构的影响并不显著(P>0.05)。Fertilization plays important roles in sustaining crop yield and quality,and soil microbial community and composition. However,little is known about the soil fungal communities in tea plantation soils,even though tea plantations constitute significant land use in South China. The diversity and distribution of soil fungal communities are significantly influenced by soil physical-chemical properties and field management. Therefore,this study sampled the surface layer( 0—10 cm) and the sub-surface layer( 10—20 cm) soils from a tea plantation in the Puer area,Yunnan Province,China. The experiment consisted of three different treatments. These were no N application( CK),chemical N fertilization with 300 kg/hm^2 N( N300),and combined inorganic and organic fertilization( 30% N in organic form,OM30). Soil DNA was extracted from the soils for PCR amplification and high throughput sequencing in order to study the effects of organic and inorganic fertilization on the fungal communities of the tea garden soil. The results showed that the surface layer( 0—10 cm)α-diversity of the fungal community,the Chao1 richness index,and the Shannon diversity index values were significantly higher than those recorded for the sub-surface layer( 10—20 cm)( P < 0. 05). Within the same layer,the α-diversity tendency was CK > N300 > OM30. Relative abundance at the phylum level showed that Ascomycota,Basidiomycota,and Zygomycota were the three dominant phyla in the experimental soil and Sordariomycetes, Tremellomycetes, and Mortierellomycotina were the three dominant Ascomycota,Basidiomycota,and Zygomycota classes,respectively. The relative abundances of Ascomycota and Basidiomycota were higher in the surface layer,whereas Zygomycota were relatively more abundant in the sub-surface layer( P < 0.05). The N300 and OM30 treatments increased the relative abundance of Zygomycota,but decreased the relative abundance of Ascomycota within the same layer. Redundancy analysis( RDA)showed that the compositions of the soil fungal communities in t
分 类 号:S571.1[农业科学—茶叶生产加工]
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