长链非编码RNA MIAT在不同分子亚型乳腺癌中的表达特点及与临床特征的关系研究  

The Expression of Long Non-coding RNA MIAT in Breast Cancer with Different Molecular Subtypes and Its Relationships with Clinical Characteristics

作　　者：吴艾冉 吴景[1] 李晓洁[1] 张兆璇 王悦 何杰[1] WU Ai-ran;WU Jing;LI Xiao-jie;ZHANG Zhao-xuan;WANG Yue;HE Jie(Department of Pathology,Anlaui Cancer Hospital,Hefei 230001,China)

机构地区：[1]安徽省肿瘤医院病理科,安徽合肥230001

出　　处：《标记免疫分析与临床》2018年第11期1703-1706,1739,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探究长链非编码RNA(long noncoding RNA,lncRNA)MIAT在乳腺癌不同分子亚型中的表达情况,并分析其表达水平与乳腺癌临床病理特征之间的关系。方法收集2015年5月至2017年9月在我院确诊的212名乳腺癌患者的乳腺癌组织、癌旁组织与外周血,行实时荧光定量PCR(quantitative real-time PCR,RT-PCR)检测组织与血浆中lncRNA MIAT表达水平。比较lncRNA MIAT在癌与癌旁组织中的表达水平,并分析其与乳腺癌分子分型、TNM分期、肿瘤直径、腋窝淋巴转移数目等临床病理特征之间的关系。结果 lncRNA MIAT在乳腺癌组织中的表达水平(4.23±1.06)明显高于癌旁组织(2.14±0.63),且差异具有统计学意义(P<0.05)。此外,癌组织与血浆中lncRNA MIAT表达水平与肿瘤分子分型、TNM分期、肿瘤直径、腋窝淋巴结转移数目等均显著相关(P <0.05),而与患者年龄、BMI、吸烟史、饮酒史、生育史、哺乳史等因素无关(P>0.05)。结论 lncRNA MIAT表达水平与乳腺癌分子分型、TNM分期、肿瘤直径及腋窝淋巴结转移数等均显著相关,对患者的预后评估具有潜在价值。Objective To investigate the expression of long non-coding RNA( lncRNA) MIAT in different molecular subtypes of breast cancer, and to analyze the relationships between its expression and the clinical features of breast cancer. Methods The breast cancer tissue, para cancer tissue and peripheral blood of 212 patients with breast cancer diagnosed in our hospital from May 2015 to September 2017 were collected and the expressions of IncRNA MIAT in tissue and plasma were detected by quantitative real-time quantitative PCR(RT-PCR). The expression levels of IncRNA MIAT in cancer and paracancerous tissues were compared,and the relationships between its expression and molecular types, TNM stages, tumor size, number of axillary lymph nodes and other clinicopathological characteristics were analyzed. Results The expression level of lncRNA MIAT in breast cancer tissues(4. 23 ± 1. 06) was significantly higher than that of para cancerous tissue(2.14 ±0. 63)(P <0. 05). In addition, the expression levels of lncRNA MIAT in cancer tissues and plasma were significantly correlated with the tumor molecular classification,TNM stages,tumor diameter,and the number of axillary lymph node metastases(P <0.05),but not related to the patient’s age,BMI,smoking history,drinking history,birth history,breast feeding history(P >0. 05). Conclusion The expression level of lncRNA MIAT was significantly related to the molecular classification of breast cancer, TNM stage, tumor diameter and the number of axillary lymph node metastases, which are of great value for the prognosis of patients.

关 键 词：乳腺癌 长链非编码RNA MIAT 分子分型 临床特征 

分 类 号：R737.9[医药卫生—肿瘤]