偶氮染料脱色菌群的脱色效果及机制研究  

Decolorization Effect of Decolorizing Azo Dye Bacterial Consortium and Their Decolorizing Mechanism

作　　者：周思懿 郭光 田芳 谢静 丁克强 杨凤 刘廷凤 胡志新 孔维杰 刘翀[3] ZHOU Siyi;GUO Guarng;TIAN Fang;XIE Jing;DING Keqiang;YANG Feng;LIU Tingfeng;HU Zhixin;KONG Weijie;LIU Chong(College of Environmental Engineering,Nanjing Institute of Technology,Nanjing 211167,China;State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,Nanjing 210008,China;Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]南京工程学院环境工程学院,江苏南京211167 [2]中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,江苏南京210008 [3]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《环境科学与技术》2018年第11期81-86,共6页Environmental Science & Technology

基　　金：国家自然科学基金项目(31600091;51608257);中国科学院饮用水科学与技术重点实验室开放基金课题(17K01KLDWST);环境模拟与污染控制国家重点联合实验室开放基金课题(18K01ESPCT);南京工程学院"人才引进项目"(YKJ201528);南京工程学院大学生科技创新项目(TB201801211);湖泊与环境国家重点实验室开放基金(2016SKL009)

摘　　要：收集江苏某印染废水中活性污泥中的酸性大红GR的脱色菌群,并使之富集,旨在提高降解偶氮染料的能力。研究结果表明,该菌群在4 h内将100 mg/L的酸性大红GR脱色96.1%。高通量测序的结果表明,该菌群主要由Alcaligenes、Pseudochrobactrum、Arthrobacter组成,其中Alcaligene和Arthrobacter是主要的脱色菌。NaNO_3、Na_2SO_4、NaCl抑制了该菌群的降解,NaNO_3的抑制作用最强,抑制率为95.1%。随着酸性大红GR的起始浓度升高,菌群的脱色率下降。该菌群在偏碱性环境下的脱色效果大于酸性环境,并表现出高效广谱染料脱色降解特性,可以脱色活性艳红X-3B、直接耐黑G和分散深蓝S-3BG等偶氮染料。GC-MS的结果表明,酸性大红GR在偶氮还原酶的作用下,偶氮键断裂,降解为苯胺和苯二胺。因此,该菌群具有高效降解偶氮染料的能力。The acid scarlet GR flora from the activated sludge in certain printing and dyeing wastewater in Jiangsu were collected and enriched, so as to improve the ability to degrade the azo dyes. The results suggested that, such flora could decolorize 96.1% of 100 mg/L acid scarlet GR within 4 h. High-throughput sequencing results indicated that such flora were mainly constituted by Alcaligenes, Pseudochrobactrum and Arthrobacte, among which, Alcaligene and Arthrobacter were the main decolorizing bacteria. NaNO3, Na2 SO4, and NaCl could inhibit the degradation of such flora, and NaNO3 had exerted the strongest inhibitory effect, with the inhibition rate of 95.1%. With the increase in the initial concentration of acid scarlet GR, the decolorizing rate of such flora was decreased. Besides, the decolorizing effect of such flora in the alkaline environment was greater than that under acidic environment. Importantly, such flora had exhibited the efficient and broad-spectrum dye decolorizing degradation characteristics, which could decolorize the azo dyes, such as reactive brilliant red X-3 B, direct fast black G,and disperse deep blue S-3 BG. The results of GC-MS showed that the azo bond was ruptured and degraded into aniline and phenylenediamine under the action of azo reductase. Therefore, such flora have potential application value in treating printing and dyeing wastewater under high salinity environment.

关 键 词：酸性大红GR 脱色 菌群 偶氮染料 

分 类 号：X170[环境科学与工程—环境科学]