桦木酸改善非酒精性脂肪肝的研究  

Study of betulinic acid to improve non-alcoholic fatty liver disease

作　　者：童蕾 汪龙[1,2,3] 金丽娜 姚霜霜[1,2,3] 杨键 张志国 Tong Lei;Wang Long;Jin Lina;Yao Shuangshuang;Yang Jian;Zhang Zhiguo(Department of Endocrinology and Metabolism,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China;Shanghai Institute of Endocrine and Metabolic Diseases,Shanghai 200025,China;Shanghai Clinical Center for Endocrine and Metabolic Diseases,Shanghai 200025,China)

机构地区：[1]上海交通大学医学院附属瑞金医院内分泌代谢病科,200025 [2]上海市内分泌代谢病研究所,200025 [3]上海市内分泌代谢病临床医学中心,200025

出　　处：《中华内分泌代谢杂志》2018年第11期939-945,共7页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金项目(81570719、81770863).

摘　　要：目的探讨桦木酸对非酒精性脂肪肝的改善作用及其机制。方法运用高脂小鼠模型,给予对照高脂饲料和含有桦木酸的高脂饲料喂食2个月。在此期间定期监测体重,并运用代谢监测系统监测两组小鼠相应代谢指标的变化。在处死小鼠后,对血清和组织中与肝脏相关的指标进行了分子生物学分析。结果给予桦木酸处理的高脂小鼠组的体重明显减轻,非酒精性脂肪肝明显改善,各种血清指标以及肝脏组织指标也有相当程度的改善,代谢率明显升高,脂肪酸合酶(fatty acid synthase,FAS)在基因水平和蛋白水平均明显下调,FAS活性也明显下调,其中普通高脂小鼠和桦木酸组高脂小鼠肝脏FAS活性分别为(1873.6±85.7)和(1181.6±85.7)pmol NADPH/min/mg protein。结论桦木酸可以从表达和活性两方面抑制脂肪合成的关键基因FAS,改善肝脏脂质沉积。Objective To explore the effect of betulinic acid on NAFLD and its mechanism.Methods We used the high-fat diet animal models,with or without feeding the standard chow diet containing betulinic acid for 2 months.During this period,the body weight was monitored regularly and metabolism cage was used to monitor the energy metabolism of the animals.After killing the mice,molecular biological analysis was performed on serum and tissue related to liver.Results In diet induced obese mice animal experiments,the mice body weight had been reduced and NAFLD had been improved significantly by betulinic acid.The various indexs of serum and liver tissue had also been significantly improved.The metabolic rate increased significantly.Fatty acid synthase gene and protein levels were significantly lower.Furthermore,FAS activity was significantly lower than the control mice.Liver FAS activity of the high fat mice and the high fat mice treated with betulinic acid were (1873.6±85.7)and (1181.6±85.7)pmol NADPH/min/mg protein,respectively.Conclusion Betulinic acid inhibited FAS at expression and activity level,and improved lipid deposition in liver.

关 键 词：高脂饮食 肥胖小鼠 非酒精性脂肪肝 脂肪酸合酶 

分 类 号：R285.5[医药卫生—中药学]