脐疗对寒凝血瘀型痛经大鼠扭体反应及神阙穴区TRPV1的影响  

Effect of navel therapy on twisting response and TRPV1 expression in Shenque of coagulated cold and blood stasis type dysmenorrhea model rats

作　　者：潘亚辉[1] 李姝婧[1] 马玉宁 马玉侠[1] PAN Ya-hui;LI Shu-jing;MA Yu-ning;MA Yu-xia(Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《时珍国医国药》2018年第10期2556-2559,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81373721);山东中医药大学创新基金课题(2018-220315)

摘　　要：目的观察脐疗对寒凝血瘀型痛经大鼠扭体反应及神阙穴区TRPV1表达的影响,初步探索脐疗效应与神阙穴区的关系及启动机制。方法将50只雌性大鼠随机分为正常对照组、模型组、药物贴脐组、隔药灸脐组、艾条灸脐组,每组各10只。除正常对照组外,其余各组均构建寒凝血瘀型痛经模型,并给予相应的治疗。记录各组大鼠扭体反应情况,通过免疫荧光法、实时荧光定量PCR技术检测各组大鼠神阙穴区TRPV1的表达。结果与模型组比较,脐疗组的扭体潜伏期明显延长(P <0. 01),扭体次数明显减少(P <0. 01),扭体评分降低(P <0. 05或P <0. 01),隔药灸脐组在延长扭体潜伏期、减少扭体次数及降低扭体评分方面优于药物贴脐组与艾条灸脐组(P <0. 05或P <0. 01);模型组TRPV1mRNA表达较正常对照组明显升高(P <0. 01);治疗后隔药灸脐组与艾条灸脐组TRPV1mRNA表达较模型组均明显升高(P <0. 01);对TRPV1mRNA表达的调节作用,艾条灸脐组与隔药灸脐组优于药物贴脐组(P <0. 01)。结论 3种脐疗法均能缓解寒凝血瘀型痛经大鼠的扭体反应,隔药灸脐法作用最优;脐疗通过上调寒凝血瘀型痛经大鼠神阙穴区TRPV1mRNA的表达以激活腧穴功能,诱导神阙信息启动;隔药灸脐法与艾条灸脐法对神阙的激活作用优于药物贴脐法。Objective To observe the effect of navel therapy on the expression of TRPV1 in Shenque of coagulated cold and blood stasis type dysmenorrhea model rats,to explore the initiation mechanism and the relationship between the umbilical effect and Shenque. Methods Fifty female rats were randomly divided into five groups: group A(normal control group),group B(model group),group C(drug sticking navel group),group D(Herb-partitioned moxibustion on navel group),group E(moxa moxibustion navel group),10 in each group. In addition to group A,coagulated cold and blood stasis dysmenorrhea model was constructed. We gave esch group the corresponding treatment,and the writhing response of rats in each group was recorded. The expression of TRPV1 in Shenque was detected by immunofluorescence and quantitative real-time PCR. Results Compared with group B,the latency of writhing of the umbilical treatment group was significantly prolonged(P < 0. 01),the number of writhing was significantly reduced(P < 0. 01),and the writhing score was decreased(P < 0. 05 or P < 0. 01),group D was superior to group C and group E in prolonged writhing latency,reduction of writhing frequency and reduction of writhing score(P < 0. 05 or P <0. 01). The expression of TRPV1 mRNA in group D and group E were significantly higher than that in group B(P < 0. 01). For the regulation of TRPV1 mRNA expression,group D and group E were superior to group C(P < 0. 01). Conclusion The three kinds of umbilical therapy all can relieve the writhing response of coagulated cold and blood stasis dysmenorrhea rats,herb-partitioned moxibustion on navel therapy has the best effect. Umbilical therapy activates the acupoint function by up-regulating the expression of TRPV1 mRNA in the Shenque of coagulated cold and blood stasis type dysmenorrhoea rat,inducing Shenque information start,the activation of Herb-partitioned moxibustion on navel therapy and and moxa moxibustion navel therapy to Shenque were better than drug sticking navel therapy.

关 键 词：脐疗 寒凝血瘀 原发性痛经 TRPV1 

分 类 号：R03[医药卫生]