补阳还五汤对脊髓损伤大鼠红核神经元L-型钙通道的影响  

Effect of Buyang Huanwu Decoction(补阳还五汤) on L-type Calcium Channel of Red Nucleus Neurons in Rats with Spinal Cord Injury

作　　者：范瑜洁 聂颖[3] 陈安[4] 殷坚[4] 杨奔[4] 李亮[5] FAN Yu-jie;NIE Yin;CHEN An;YIN Jian;YANG Ben;LI Liang(Hu'nan Brain Hospital,Changsha Hu'nan 410007,China;People's Hospital Affiliated to Hu'nan University of Chinese Medicine,Changsha Hu'nan'410007,China;The First Affiliated Hospital of Hu'nan University of Chinese Medicine,Changsha Hu'nan 410007,China;Department of Anatomy,Hu'nan University of Chinese Medicine,Changsha Hu'nan 410208,China;Provincial Key Laboratory of TCM Diagnostics,Hu'nan University of Chinese Medicine,Changsha Hu'nan 410208,China)

机构地区：[1]湖南省脑科医院,湖南长沙410007 [2]湖南中医药大学附属人民医院,湖南长沙410007 [3]湖南中医药大学第一附属医院,湖南长沙410007 [4]湖南中医药大学人体解剖学教研室,湖南长沙410208 [5]湖南中医药大学中医诊断学湖南省重点实验室,湖南长沙410208

出　　处：《中医药导报》2018年第20期26-29,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然基金资助项目(2018JJ2289);国家自然科学基金资助项目(81302899);湖南省中医药科研计划项目(2013111);湖南中医药大学中医诊断学国家重点学科开放基金(2014-37)

摘　　要：目的:探究补阳还五汤对脊髓损伤大鼠红核神经元L-型钙通道(ICa-L)电流及活动变化的影响。方法:将大鼠随机分为假手术组、模型组、补阳还五汤低、中、高剂量组,每组10只。采用红核脊髓束(rubrospinal tract,RST)横断方法,建立脊髓损伤动物模型,分离红核细胞。采用膜片钳技术记录各组ICa-L的变化及离子活动。结果:模型组红核细胞ICa-L电流密度在测试电压-20～+70 mV之间均明显低于假手术组(P<0.01),模型组曲线左移,ICa-L最大电流时的激活电压明显低于假手术组(P<0.01)。补阳还五汤低剂量组的ICa-L电流密度在测试电压20～+40 mV之间均高于模型组(P<0.05);补阳还五汤中、高剂量组的ICa-L电流密度在测试电压-10～+50mV之间均高于模型组(P<0.05或<0.01)。模型组ICa-L开放时间、开放概率均高于假手术组(P<0.05)。补阳还五汤中、高剂量组开放时间、开放概率均低于模型组(P<0.01)。结论:补阳还五汤治疗脊髓损伤可能与其抑制神经细胞ICa-L的开放防止钙超载有关。Objective:To study the effects of Buyang Huanwu Decoction (BYHWD)on L-type calcium channel (ICa-L)of Red Nucleus Neurons in rats with Spinal cord injury.Methods:The experiment rats were randomly divided into sham operation group,model group,BYHWD low groups,BYHWD mediate groups and BYHWD high groups,10rats in each group.The ruhrospinal tract was damaged in modal group and BYHWD group and given red nucleus neurons;the change of ICa-L currents were detected with Patch-clamp techniques. Results:Compared with the sham operation group,the model group showed lower ICa-L density of erythrocyte between test voltage -20mV and +70mV in Red Nucleus Neurons,and the curve of model group shifted to the left.The activation voltage at the maximum current of ICa-L was significantly lower than that of the sham operation group (P<0.01).The ICa-L current density in the BYHWD low dose group was higher than that of the model group between the test voltage 20mV and +40mV (P<0.05);The ICa-L current density of medium andhigh dose group in BYHWD was higher than that of the model group between the test voltage -10mV to + 50mV (P<0.05or P<0.01).Compared with the sham operation group,the ICa-L opening time of the model group was prolonged and the opening probability was increased (P<0.05).Compared with the model group,the opening time in the medium and high dose group of BYHWD was shortened and the opening probability decreased,which was statistically significant (P<0.01).Conclusion:BYHWD could inhibit L-type calcium channel in Red Nucleus Neurons of Spinal cord injury.It implied that BYHWD could inhibit L-type calcium channel in neuron.

关 键 词：脊髓损伤 红核 L-钙通道 补阳还五汤 大鼠 

分 类 号：R285.5[医药卫生—中药学]