Taurine Transporter dEAAT2 is Required for Auditory Transduction in Drosophila  

作　　者：Ying Sun Yanyan Jia Yifeng Guo Fangyi Chen Zhiqiang Yan 

机构地区：[1]State Key Laboratory of Medical Neurobiology,Human Phenome Institute,Ministry of Education Key Laboratory of Contemporary Anthropology,Collaborative Innovation Center of Genetics and Development,Department of Physiology and Biophysics,School of Life Sciences,Fudan University,Shanghai 200438,China [2]Department of Human Anatomy,School of Basic Medicine Sciences,Southwest Medical University,Luzhou 646000,China [3]Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Neuroscience Bulletin》2018年第6期939-950,共12页神经科学通报（英文版）

基　　金：supported by funds from The Ministry of Science and Technology of China (2017YFA0103900 and 2016YFA0502800);The National Natural Science Foundation of China (31571083);supported by The National Natural Science Foundation of China (81470701);The National Natural Science Foundation of China (81771882);The Program for Professor of Special Appointment (Eastern Scholar of Shanghai; TP2014008);The Shanghai Rising-Star Program (14QA1400800);a grant from the Young 1000 Talent Program of China to ZY;The Fundamental Research (Discipline Layout) Foundation from Shenzhen Committee of Science, Technology and Innovation (JCYJ20170817111912585) to FC

摘　　要：Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. d EAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca2+imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound.More importantly, expressing d EAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together,these findings indicate a critical role for Drosophila dEAAT2 in sound transduction by chordotonal neurons.Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. d EAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca^(2+) imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound.More importantly, expressing d EAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together,these findings indicate a critical role for Drosophila dEAAT2 in sound transduction by chordotonal neurons.

关 键 词：Drosophila dEAAT2 TAURINE Chordotonal neurons Sound transduction 

分 类 号：R[医药卫生]