Laminar Distribution of Neurochemically-Identified Interneurons and Cellular Co-expression of Molecular Markers in Epileptic Human Cortex  

作　　者：Qiyu Zhu Wei Ke Quansheng He Xiongfei Wang Rui Zheng Tianfu Li Guoming Luan Yue-Sheng Long Wei-Ping Lia Yousheng Shu 

机构地区：[1]State Key Laboratory of Cognitive Neuroscience and Learning and IDG/McGovern Institute for Brain Research,Beijing Normal University,Beijing 100875,China [2]College of Pharmaceutical Sciences,Brain Institute,Capital Medical University,Beijing 100069,China [3]Department of Neurosurgery,Epilepsy Center,Sanbo Brain Hospital of Capital Medical University,Beijing Key Labo-ratory of Epilepsy,Epilepsy Institution,Beijing Institute for Brain Disorders,Beijing 100093,China [4]Institute of Neuroscience and Department of Neurology of the Second Affiliated Hospital of Guangzhou Medical University,Key Laboratory of Neurogenetics and Chan-nelopathies of Guangdong Province and the Ministry of Education of China,Guangzhou 501260,China

出　　处：《Neuroscience Bulletin》2018年第6期992-1006,共15页神经科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31430038 and 81571275)

摘　　要：Inhibitory GABAergic interneurons are fundamental elements of cortical circuits and play critical roles in shaping network activity. Dysfunction of interneurons can lead to various brain disorders, including epilepsy,schizophrenia, and anxiety. Based on the electrophysiological properties, cell morphology, and molecular identity,interneurons could be classified into various subgroups. In this study, we investigated the density and laminar distribution of different interneuron types and the coexpression of molecular markers in epileptic human cortex.We found that parvalbumin(PV) and somatostatin(SST)neurons were distributed in all cortical layers except layer I, while tyrosine hydroxylase(TH) and neuropeptide Y(NPY) were abundant in the deep layers and white matter.Cholecystokinin(CCK) neurons showed a high density in layers IV and VI. Neurons with these markers constituted*7.2%(PV), 2.6%(SST), 0.5%(TH), 0.5%(NPY), and4.4%(CCK) of the gray-matter neuron population. Doubleand triple-labeling revealed that NPY neurons were also SST-immunoreactive(97.7%), and TH neurons were more likely to express SST(34.2%) than PV(14.6%). A subpopulation of CCK neurons(28.0%) also expressed PV, but none contained SST. Together, these results revealed the density and distribution patterns of different interneuron populations and the overlap between molecular markers in epileptic human cortex.Inhibitory GABAergic interneurons are fundamental elements of cortical circuits and play critical roles in shaping network activity. Dysfunction of interneurons can lead to various brain disorders, including epilepsy,schizophrenia, and anxiety. Based on the electrophysiological properties, cell morphology, and molecular identity,interneurons could be classified into various subgroups. In this study, we investigated the density and laminar distribution of different interneuron types and the coexpression of molecular markers in epileptic human cortex.We found that parvalbumin(PV) and somatostatin(SST)neurons were distributed in all cortical layers except layer I, while tyrosine hydroxylase(TH) and neuropeptide Y(NPY) were abundant in the deep layers and white matter.Cholecystokinin(CCK) neurons showed a high density in layers IV and VI. Neurons with these markers constituted*7.2%(PV), 2.6%(SST), 0.5%(TH), 0.5%(NPY), and4.4%(CCK) of the gray-matter neuron population. Doubleand triple-labeling revealed that NPY neurons were also SST-immunoreactive(97.7%), and TH neurons were more likely to express SST(34.2%) than PV(14.6%). A subpopulation of CCK neurons(28.0%) also expressed PV, but none contained SST. Together, these results revealed the density and distribution patterns of different interneuron populations and the overlap between molecular markers in epileptic human cortex.

关 键 词：INTERNEURON Epilepsy Human cortex Cell type IMMUNOSTAINING PARVALBUMIN Somatostatin Tyrosine hydroxylase Neuropeptide Y CHOLECYSTOKININ 

分 类 号：R[医药卫生]