唾液腺腺样囊性癌XT-Ⅰ和XT-Ⅱ基因沉默效果的比较  

Comparison of the effect of XT-Ⅰ and XT-Ⅱ gene silenced on salivary adenoid cystic carcinoma

作　　者：徐明 王洁[1] 刘慧娟 张艳宁[1] 董福生[2] XU Ming;WANG Jie;LIU Huijuan;ZHANG Yanning;DONG Fusheng(Department of Oral Pathology College and Hospital of Stomatology Key Laboratory of Stomatology Hebei Medical University,Shijiazhuang 050017)

机构地区：[1]河北医科大学口腔医学院·河北省口腔医学重点实验室口腔病理科,石家庄050017 [2]河北医科大学口腔医学院口腔颌面外科,石家庄050017

出　　处：《现代口腔医学杂志》2018年第6期325-331,共7页Journal of Modern Stomatology

基　　金：国家自然基金资助(81170976)

摘　　要：目的比较唾液腺腺样囊性癌XT-Ⅰ基因和XT-Ⅱ基因沉默后,蛋白多糖PGs分泌阻抑的效率。方法构建针对靶向沉默XT-Ⅰ、XT-Ⅱ基因的腺病毒载体,转染SACC-83细胞,沉默XT-Ⅰ基因和XT-Ⅱ基因。采用Real-time PCR、Western blot检测XT-Ⅰ和XT-Ⅱ基因和蛋白的表达,通过Blyscan Assay Kit检测蛋白多糖PGs阻抑的效率。结果(1)XT-Ⅰ基因和XT-Ⅱ基因mRNA表达分别减少64%和54%。(2)XT-Ⅰ、XT-Ⅱ蛋白的相对表达量分别减少38%和31%。(3)蛋白多糖合成量分别降低49.69%和36.47%,差别有显著性(P<0.05)。结论 (1)分别沉默XT-Ⅰ、XT-Ⅱ基因均能有效抑制SACC-83细胞蛋白多糖的生物合成。(2)沉默XT-Ⅰ基因,阻抑蛋白多糖合成量效率(49.69%)明显高于XT-Ⅱ基因(36.47%)。Objective To compare the efficiency of proteoglycans (PGs)inhibition by XT-Ⅰ and XT-Ⅱ gene silenced in adenoid cystic carcinoma of salivary gland.Methods Recombinant adenovirus vector for silencing XT-Ⅰ gene and XT-Ⅱ gene was constructed and transfected SACC-83cells respectively.XT-Ⅰ and XT-Ⅱ gene and protein expression was detected by Real-time PCR and Western blot.The efficiency of PGs inhibition was evaluated by Blyscan Assay Kit.Results (1)The mRNA expression of XT-Ⅰ and XT-Ⅱ genes was decreased by 64%and 54%, respectively.(2)The relative expression of XT-Ⅰ and XT-Ⅱ protein was decreased by 38%and 31%,respectively. (3)The proteoglycan contents were decreased by 49.69%and 36.47%,respectively,the difference was significant (P<0.05).Conclusion (1)Both XT-Ⅰ gene or XT-Ⅱ gene was silenced,the biosynthesis of PGs in SACC-83cells could be suppressed significantly.(2)The inhibitory efficiency of XT-Ⅰ gene (49.69%)was significantly higher than that of XT-Ⅱ gene (36.47%).

关 键 词：腺样囊性癌 蛋白多糖 木糖基转移酶-Ⅰ 木糖基转移酶-Ⅱ 唾液腺肿瘤 

分 类 号：R780.2[医药卫生—口腔医学]