基质衍生因子1α及骨保护蛋白表达与多发性骨髓瘤骨病的相关性  

Correlation between expressions of stromal cell-derived factor 1α and osteoprotegerin and bone disease in multiple myeloma

作　　者：高大[1] 苏伊拉[1] 李慧娉[1] 韩东海 张亚朋 骈晓琴 Gao Do;Su Yilo;Li Huipin;Han Donghai;Zhang Yapeng;Pian Xiaoqin(Department of Hematology,the Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院血液内科,呼和浩特010050

出　　处：《白血病．淋巴瘤》2018年第11期651-655,共5页Journal of Leukemia & Lymphoma

基　　金：内蒙古自治区自然科学基金(2016MS08146).

摘　　要：目的探讨多发性骨髓瘤(MM)伴或不伴骨髓瘤骨病(MBD)患者血清基质衍生因子1α(SDF-1α)、骨保护蛋白(OPG)与β2微球蛋白(β2-MG)表达水平的相关性。方法选取内蒙古医科大学附属医院2014年1月至2017年6月收治的MM患者80例,均符合国际MM诊断标准。依据骨痛等症状分为伴MBD组45例、不伴MBD组35例。分别采用酶联免疫吸附试验(ELISA)检测患者血清SDF-1α与OPG水平,放射免疫法检测MM主要预后指标β2-MG水平。对采用随机数字表法选择的45例患者骶髂关节行X线检查后,进行三维骨重建,行MBD评分。组间分类变量比较采用X^2检验,符合正态分布的连续变量两组间比较采用两独立样本t检验,相关性分析采用Pearson法。结果伴MBD组MM患者血清SDF-1α水平较不伴MBD组患者升高[(0.31±0.17)pg/ml比(0.18±0.06)pg/ml],差异有统计学意义(t=-4.21,P<0.001);伴MBD组患者血清OPG水平较不伴MBD组降低[(0.73±0.50)pg/ml比(1.08±0.31)pg/ml],差异有统计学意义(t=3.62,P<0.001)。Pearson分析结果显示,伴MBD组患者β2-MG水平与SDF-1α水平呈正相关(r=0.84,P<0.001),与OPG水平呈负相关(r=-0.48,P<0.001);不伴MBD组患者β2-MG水平与SDF-1α、OPG水平均未显示有相关性(均P>0.05)。结论MM伴MBD患者血清中β2-MG与SDF-1α表达水平升高,OPG水平降低,SDF-1α、OPG可为作为MBD诊断、治疗及预后评估新的临床生化指标。Objective To understand the correlation of expression levels of serum stromal cellderived factor 1α (SDF-1α),osteoprotegerin (OPG)and β2 microglobulin (β2-MG)in patients with multiple myeloma (MM)with or without myeloma bone disease (MBD).Methods Eighty.patients with MM who were admitted to the Affiliated Hospital of Inner Mongolia Medical University from January 2014to June 2017were selected;all of the patients met the international diagnostic criteria for MM.According to the symptoms such as bone pain,the patients were divided into group with MBD (45cases)and group without MBD (35cases). Enzyme-linked immunosorbent assay (ELISA)was used to detect the serum levels of SDF-1α and OPG,and radioimmunoassay was used to detect the expression of MM major prognostic indicator β2-MG.The MBD score was evaluated in 45 patients selected by random number table after sacroiliac joint X-ray and threedimensional bone reconstruction.The X^2 test was used to compare the categorical variables;the two independent sample t-test was used to compare the continuous variables that conformed to the normal distribution between two groups,and the Pearson method was used for the correlation analysis.Results The expression level of serum SDF-1α in the group with MBD was significantly higher than that in the group without MBD [0.31±0.17)pg/ml vs.(0.18±0.06)pg/ml],and the difference was statistically significant (t = -4.21,P <0.001).The expression level of serum OPG in the group with MBD was significantly lower than that in the group without MBD [(0.73±0.50)pg/ml vs.(1.08±0.31)pg/ml],and the difference was statistically significant (t =3.62,P <0.001).Pearson analysis showed that β2 -MG level in the group was positively correlated with SDF-1α level (r =0.84,P <0.001),and negatively correlated with OPG level (r =-0.48,P < 0.001).The β2-MG level in the group without MBD did not show a correlation with the SDF-1α and OPG levels.Conclusions In the serum of patients with MBD,the expression levels of β2-MG and SDF-1α are increased,an

关 键 词：多发性骨髓瘤 骨髓瘤骨病 基质衍生因子 骨保护蛋白 Β2微球蛋白 

分 类 号：R733.3[医药卫生—肿瘤]