猴头菌-青蒿生物转化物的化学成分研究  

Chemical Constituents from the Biological Transformation of Hericium erinaceus-Artemisia annua

作　　者：徐博[1] 李娟[1,2] 赵小芳 杨蓉[1] 何述金 李顺祥 XU Bo;LI Juan;ZHA0Xiao-fang;YANG Rong;HE Shu-jin;LI Shun-xiang(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine,Changsha 410208,China;Hunan Xinhui PharmaeyCo.,Ltd.,Chang-sha 410200,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湖南省中药活性物质筛选工程技术研究中心,湖南长沙410208 [3]湖南新汇制药股份有限公司,湖南长沙410200

出　　处：《中药材》2018年第3期613-616,共4页Journal of Chinese Medicinal Materials

基　　金：中药学湖南省重点学科科技项目(zy201501);湖南省研究生科研创新项目(CX2015B323)

摘　　要：目的:研究猴头菌-青蒿生物转化物的化学成分。方法:采用硅胶柱层析、Sephadex LH-20凝胶柱色谱及重结晶等方法分离纯化,根据化合物的理化性质、波谱数据鉴定化学结构。结果:从猴头菌-青蒿生物转化物中分离得到10个化合物,分别鉴定为:麦角甾-7,22-二烯-3-酮(1)、豆甾-4-烯-6α-醇-3-酮(2)、(24R)-5α-豆甾-3,6-二酮(3)、fortisterol(4)、β-谷甾醇(5)、莨菪亭(6)、异茴芹内酯(7)、比克白芷素(8)、旱前胡乙素(9)、白花前胡丁素(10)。结论:其中,化合物2、4、8～10为在猴头菌丝体和青蒿的化学成分报道中首次发现,为生物转化后产物,此研究为进一步探讨猴头菌-青蒿经生物转化后成分组成的变化提供依据。Objective:To investigate the chemical constituents from the biological transformation of Hericium erinaceus-Artemisia annua.Methods:The chemical constituents were separated and purified with silica gel,Sephadex LH-20 gel.And their structures were elucidated by physiochemical properties,spectroscopic analysis.Results:Ten compounds have been isolated from the biological transformation of Hericium erinaceus and Artemisia annua,and identified as ergosta-7,22-dien-3-one(1),stigmasta-4-ene-6α-ol-3-one(2),(24R)-5α-stigmasta-3,6-dione(3),fortisterol(4),β-sitosterol(5),scopoletin(6),isopimpinellin(7),byakangelicin(8),daucoidin B(9),praeruptorin D(10).Conclusion:Compounds 2、4、8~10 are firstly obtained from Hericium erinaceus and Artemisia annua,and indicats that it is the bioconversion products,which provide the basis for exploring the chemical constituents changes of the culture of Hericium erinaceus and Artemisia annua further.

关 键 词：生物转化物 猴头菌 青蒿 化学成分 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]