CD-105与Ki-67在喉鳞状细胞癌组织中的表达及相关性研究  

Expression and correlation of CD-105 and Ki-67 in laryngeal squamous cell carcinooma

作　　者：狄国华 高英恺[1] 仲云[1] 杨凡[1] 王彬[1] 曹艳丽[1] DI Guohua;GAO Yingkai;ZHONG Yun;YANG Fan;WANG Bin;CAO Yanli(Department of Otorhinolaryngology,Weifang Hospital of Traditional Chinese Medicine,Weifang 261041.,China)

机构地区：[1]潍坊市中医院耳鼻喉科,山东潍坊261041

出　　处：《潍坊医学院学报》2018年第5期388-390,F0004,共4页Acta Academiae Medicinae Weifang

基　　金：潍坊市科技局科研项目计划(项目编号:2016YX049)

摘　　要：目的通过研究CD105与Ki-67在喉鳞状细胞癌组织中的表达与肿瘤的组织分化程度、临床分期及颈部淋巴结是否转移之间的关系,探讨CD105与Ki-67评价喉鳞状细胞癌发生、发展及临床预后的意义。方法取喉癌患者手术所留取的标本61例,声门上型28例,声门型30例,声门下型3例,选取正常喉部组织20例作为对照组1,选取癌肿旁切缘即癌旁组织作为照组2,应用免疫组化S-P法检测CD105及Ki-67的表达。比较CD105-MVD值及Ki-67基因蛋白在喉鳞状细胞癌、正常喉组织与癌旁组织的标记程度。结果喉鳞状细胞癌、正常喉组织与癌旁组织CD105-MVD分别为7. 63±1. 79,1. 30±1. 00,3. 80±2. 00,3组间差异有显著性(P <0.01); CD105标记的MVD值过度表达与癌肿细胞的分化程度无统计学相关性(P> 0. 05),与癌肿的临床分期及淋巴结的转移有显著相关关系(P <0. 05)。Ki-67阳性表达在喉鳞状细胞癌、正常喉组织与癌旁组织表达分别为78. 7%(48/61),0%(0/20),3. 3%(2/61),差异有显著性(P <0. 01); Ki-67旳过度表达与肿瘤的组织分化程度无统计学相关性(P> 0. 05); Ki-67蛋白表达与患者恶性肿瘤的临床分期以及颈部淋巴结转移是否转移有显著相关性(P <0. 05)。结论 CD105与Ki-67在喉鳞状细胞癌高表达,与肿瘤的组织分化程度无统计学相关性,与临床分期及颈部淋巴结是否转移有显著相关性;喉鳞状细胞癌CD105-MVD值与Ki-67表达有明显相关性。Objective To explore the significance of CD105 and Ki-67in evaluating the development and clinical prognosis of laryngeal squamous cell carcinoma,through the study of CD105 and Ki-67expression in laryngeal squamous cell carcinoma tissue and their relationship among the expression and tumor tissue differentiation,clinical staging and cervical lymph node metastasis.Methods There were 61cases of patients with laryngeal cancer,including 28cases of supraglottic,30cases of glottic ,and 3cases of subglottic.20cases of normal laryngeal tissue were selected as control group 1.The adjacent margin of cancer was selected as group 2.The expression of CD105and Ki-67 was detected by immunohistochemical s-p,the degrees of markedness of CD105-MVD value and Ki-67gene protein in laryngeal squamous cell carcinoma,normal laryngeal tissue and adjacent tissue were compared.Results Laryngeal squamous cell carcinoma,normal laryngeal tissue and adjacent tissue CD105-MVD were 7.63±1.79,1.30±1.00,3.80±2.00.There was significant difference among the three groups (P < 0.01). There was no statistical correlation between excessive expression of CD105-MVD value and the degree of differentiation of cancer cells,which had significant correlation with the clinical stages of cancer and lymph node metastasis (P <0.05).The expression of Ki-67positive expression was 78.7%,0%and 3.3%,respectively,in laryngeal squamous cell carcinoma,normal laryngeal tissue and carcinoma,and the difference was significant(P <0.01).There was no statistically significant correlation between the overexpression of Ki-67and tumor tissue differentiation(P >0.05).The expression of Ki-67protein was significantly correlated with the clinical stage of tumor and metastasis of cervical lymph nodes (P <0.05).Conclusions The high expression of CD105and Ki-67in laryngeal squamous cell carcinoma shows no statistical correlation with tumor tissue differentiation,and is significantly correlated with clinical staging and cervical lymph node metastasis. There is a significant correlation

关 键 词：喉鳞状细胞癌 KI-67 CD105 

分 类 号：R739.65[医药卫生—肿瘤]