熊果酸拮抗巨噬细胞促进肝癌细胞迁移和侵袭的作用及其机制  

Effect of ursolic acid on invasion and migration of hepatocellular carcinoma cells co-cultured with macrophages and the underlying mechanisms

作　　者：李瑶瑶 申恩华[2] LI Yaoyao;SHEN Enhua(Medical College,Jingchu University of Technology,Jingmen Hubei 448000;Department of Infection,Fourth Hospital of Jilin University,Changchun 130000,China)

机构地区：[1]荆楚理工学院医学院,湖北荆门448000 [2]吉林大学第四医院感染科,长春130000

出　　处：《中南大学学报（医学版）》2018年第11期1188-1193,共6页Journal of Central South University ：Medical Science

基　　金：湖北省教育厅科学技术研究项目(Q20184303)~~

摘　　要：目的:研究熊果酸对巨噬细胞-肝癌细胞共培养体中肝癌细胞迁移和侵袭能力的影响,并初步探讨其作用机制。方法:采用Transwell迁移和侵袭试验检测熊果酸处理与非处理的巨噬细胞-肝癌细胞共培养体中肝癌细胞的迁移和侵袭能力的变化;Western印迹法检测共培养体中肝癌细胞上皮-间质转化蛋白E-cadherin,N-cadherin和vimentin的表达情况。结果:共培养后的肝癌细胞的迁移和侵袭能力显著增强,并发生了上皮-间质样表型转变,上皮-间质转化的标志蛋白E-cadherin的表达下调,而N-cadherin和vimentin的表达均上调;熊果酸处理的巨噬细胞-肝癌细胞共培养体中,肝癌细胞的迁移和侵袭能力降低,E-cadherin表达上调,N-cadherin和vimentin的表达下调。结论:熊果酸能够显著抑制共培养体中肝癌细胞的迁移和侵袭能力及上皮-间质转化能力。Objective:To investigate the effect of ursolic acid on the invasion and migration ofhepatocellular carcinoma (HCC)cells co-cultured with macrophages,and to explore the underlying mechanisms. Methods.The migration and invasion ability of HCC cells in the co-culture system with or without ursolic acid intervention were evaluated by transwell assay.The levels of epithelial-mesenchymal transition (EMT)markers E-cadherin,N-cadherin,and vimentin in HCC cells co-cultured with macrophages were detected by Western blot. Results:The migration and invasion ability and EMT were significantly enhanced when cocultured with macrophages,and the expression of E-cadherin was significantly increased while N-cadherin and vimentin levels were significantly decreased.However,after ursolic acid treatment, the migration and invasion ability were significantly reduced,and the expression of E-cadherin was increased while N-cadherin and vimentin levels were decreased. Conclusion:Ursolic acid exerts inhibitory effect on the ability of migration,invasion,and EMT for HCC,which are enhanced by co-culturing with macrophages.

关 键 词：肝癌细胞 巨噬细胞 共培养 熊果酸 迁移 侵袭 上皮-间质转化 

分 类 号：R735.7[医药卫生—肿瘤]