Wnt5a对甲状腺乳头状癌细胞增殖及侵袭能力的影响  

The effect of wnt5a on proliferation and invasion of papillary thyroid carcinoma cells

作　　者：陈亮[1] 冀宏[2] 石东亮[1] 刘肖俊 Chen Liang;Ji Hong;Shi Dongliang(The Second Department of Thyroid and Breast Surgery,the Center Hospital of Cangzhou,Hebei Medical University,Cangzhou 061001,China;Department of General Surgery,the 2nd Hospital of Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北省沧州市中心医院甲状腺乳腺外二科,沧州061001 [2]河北医科大学第二医院普通外科,石家庄050017

出　　处：《中华实验外科杂志》2018年第12期2222-2225,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察Wnt5a表达对甲状腺乳头状癌细胞生物学行为的影响。方法噻唑蓝(MTT)法检测敲低或过表达Wnt5a对甲状腺乳头状癌细胞增殖活性的影响。克隆形成实验检测敲低或过表达Wnt5a对甲状腺乳头状癌细胞单个细胞克隆形成能力的影响。Transwell实验检测敲低或过表达Wnt5a对甲状腺乳头状癌细胞侵袭能力的影响。结果MTT法实验显示,Wnt5a过表达的K1细胞在3、4d检测到的吸光度(A)值(0.403±0.003、0.562±0.005)均大于对照组(0.371±0.006、0.454±0.002),Wnt5a-小干扰RNA(siRNA)-2组A值(0.342±0.005、0.402±0.004)均小于对照组,差异有统计学意义(t=0.951、14.206、-6.431、-20.140,P=0.030、0.000、0.003、0.000)。Wnt5a过表达BCPAP细胞在3、4d检测到的A值(0.454±0.007、0.603±0.002)均大于对照组(0.403±0.006、0.493±0.002),Wnt5a-siRNA-2组A值(0.362±0.005、0.370±0.003)均小于对照组,差异有统计学意义(t=0.839、27.361、-9.092、-29.087,P=0.010、0.000、0.001、0.000)。平板克隆形成实验显示,过表达Wnt5a后,细胞株K1及BCPAP细胞克隆的形成数[(130.00±4.34)、(117.00±3.54)个]均明显高于对照组[(88.00±3.44)、(86.00±3.48)个],敲低Wnt5a后,K1及BCPAP细胞克隆的形成数[(50.00±3.45)、(47.00±4.01)个]均明显低于对照组,差异均有统计学意义(t=13.136、10.816、-13.510、-12.253,P=0.000)。Transwell实验显示,过表达Wnt5a后,与对照组[(80.20±1.23)、(83.90±1.45)个]比较,细胞株K1及BCPAP每个高倍镜视野中穿过基底膜细胞数[(155.60±2.01)、(164.50±2.09)个]明显增多,差异均有统计学意义(t=-25.420、-24.881,P=0.000)。敲低Wnt5a后,与对照组比较,细胞株K1及BCPAP穿过基底膜细胞数[(50.80±1.02)、(52.30±1.08)个]明显减少,差异均有统计学意义(t=21.868、28.938,P=0.000)。结论过表达Wnt5a能够促进甲状腺乳头状癌细胞的增殖、克隆和侵袭;敲低Wnt5a能够抑制细胞的增殖、克隆和侵袭。Objective To investigate the effect of Wnt5a expression on the biological behavior of papillary thyroid carcinoma cells. Methods The proliferation of papillary thyroid carcinoma cells with Wnt5a low-expressed or over-expressed was detected by MTT. The clone-forming abilities of papillary thyroid carcinoma cells with Wnt5a low-expressed or over-expressed were detected by colony formation assays. The invasion abilities of papillary thyroid carcinoma cells with Wnt5a low-expressed or over-expressed were detected by Transwell assays. Results The MTT assays demonstrated that A values of Wnt5a over-expressing K1 cells detected on the third and fourth days (0.403±0.003, 0.562±0.005) were significantly higher than those of the control groups (0.371±0.006, 0.454±0.002), and the A values of the Wnt5a-small interfering RNA (siRNA)-2 groups (0.342±0.005, 0.402±0.004) were significantly lower than those of the control groups (t=0.951, 14.206, -6.431, -20.140; P=0.030, 0.000, 0.003, 0.000). Besides, the A values of Wnt5a over-expressing BCPAP cells detected on the third and fourth days (0.454±0.007, 0.603±0.002) were significantly higher than those of the control groups (0.403±0.006, 0.493±0.002), and the A values of the Wnt5a-siRNA-2 groups (0.362±0.005, 0.370±0.003) were significantly lower than those of the control groups (t=0.839, 27.361, -9.092, -29.087; P=0.010, 0.000, 0.001, 0.000). The results of colony formation assays showed that the clone numbers of cell line K1 and BCPAP cells (130.00±4.34, 117.00±3.54) were significantly greater than those of the control groups (88.00±3.44, 86.00±3.48) after over-expressing Wnt5a (t=13.136, 10.816; P=0.000). Whereas, after knocking down Wnt5a, the numbers of K1 and BCPAP cell clones (50.00±3.45, 47.00±4.01) were significantly lower than those of the control groups (t=-13.510, -12.253; P=0.000). The Transwell assays showed that after over-expressing Wnt5a, compared with the control groups [(80.20±1.23), (83.90±1.45) cells], the numbers of cells passing through t

关 键 词：WntSa 甲状腺乳头状癌 细胞增殖 细胞侵袭 

分 类 号：R736.1[医药卫生—肿瘤]