稳定过表达微小RNA-637对甲状腺癌细胞生物学行为的影响  

Effect of over-expression of microRNA-637 on biological behaviors of thyroid cancer cells

作　　者：袁青领[1] 刘洋[1] 樊玉霞[1] 刘征[1] 王晓明[1] 贾勐[1] 耿祖仕[1] 卢秀波[1] Yuan Qingling;Liu Yang;Fan Yuxia;Liu Zheng;Wang Xiaoming;Jia Meng;Geng Zushi;Lu Xiubo(Department of Thyroid Surgery,the First Affiliated Hospital of Zhengzhou University,Key - Discipline Laboratory Clinical Medicine,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院甲状腺外科、河南省高等学校临床医学重点学科开放实验室,450052

出　　处：《中华实验外科杂志》2018年第12期2226-2228,共3页Chinese Journal of Experimental Surgery

基　　金：河南省医学科技攻关计划项目(201402007)。

摘　　要：目的观察过表达微小RNA(miRNA,miR)-637对甲状腺癌细胞TPC-1生物学行为的影响。方法采用慢病毒转染将过表达miR-637质粒(实验组)和空白质粒(阴性对照组)分别转入TPC-1细胞,通过细胞计数试剂盒(CCK-8)法观察细胞增殖情况,流式细胞仪分析细胞凋亡率及周期分布,划痕实验和Transwell侵袭实验评价细胞迁移和侵袭能力。结果实验组TPC-1细胞生长曲线趋势较为平缓,细胞生长速度较阴性对照组显著降低(t=5.840,P=0.028)。与阴性对照组[(12.50±1.71)%]比较,实验组[(20.75±0.93)%]TPC-1细胞的凋亡率明显增加(t=19.674,P=0.003)。与阴性对照组细胞比较,实验组细胞在48h后S期下降至23.35%,G2/M期下降至17.58%。划痕实验及Transwell侵袭实验表明,与阴性对照组细胞比较,实验组的细胞迁移数明显减少(t=-26.186,P=0.001)。结论过表达miR-637能降低TPC-1细胞的增殖、凋亡、迁移和侵袭能力。Objective To observe the effect of over-expression of microRNA (miRNA, miR)-637 on the biological behaviors of thyroid cancer cell line TPC-1. Methods The over-expressed miR-637 plasmids (experimental group) and blank plasmid (negative control group) were transferred into TPC-1 cells respectively by lentivirus transfection. Cell proliferation was observed by cell counting kit-8 (CCK-8), cell apoptosis rate and cycle distribution were analyzed by flow cytometry, and scratch test and Transwell invasion test were used to evaluate the cell proliferation, cell migration and invasiveness. Results The growth curve of TPC-1 cells in the experimental group was relatively slow, and the cell growth rate was significantly lower than that in the negative control group (t=5.840, P=0.028). As compared with the negative control group [(12.50±1.71)%, the apoptosis rate of TPC-1 cells in the experimental group [(20.75±0.93)%] was significantly increased (t=19.674, P=0.003). The ratio of cell apoptosis was significantly higher in the experimental group than that in the negative control group. As compared with the experimental group, the percentage of cells in S phase was decreased to 23.35% after 48 h and that in G2/M phase decreased to 17.58%. Scratch test and Transwell invasion test showed that the number of migrating cells in the experimental group decreased significantly as compared with the negative control group (t=-26.186, P=0.001). Conclusion The over-expression of miR-637 can reduce the proliferation, apoptosis, migration and invasion of TPC-1 cells.

关 键 词：甲状腺癌 微小RNA-637 增殖 凋亡 迁移 侵袭 

分 类 号：R736.1[医药卫生—肿瘤]