一种利用不同斑纹品种混精杂交筛选转基因家蚕纯合子的方法  

A Method for Screening Transgenic Homozygote by Mixed Sperm Fertilization of Different Marking Varieties in Bombyx mori

作　　者：张雨丽 赵爱春[2] 龙定沛[2] 闭立辉 韦伟 鲁成[2] 张桂征 Zhang Yuli;Zhao Aiohun;Long Dingpei;Bi Lihui;Wei Wei;Lu Cheng;Zhang Guizheng(Sericultural Research Institute,Guangxi Zhuang Autonomous Region,Nanning 530007,China;Institute of Sericulture and Systems Biology,Southwest University,Chongqing 400716,China)

机构地区：[1]广西壮族自治区蚕业科学研究院,南宁530007 [2]西南大学蚕学与系统生物学研究所,重庆400716

出　　处：《蚕业科学》2018年第5期805-809,共5页ACTA SERICOLOGICA SINICA

基　　金：广西科学研究与技术开发计划项目(No.桂科合1599005-2-16);广西第五批特聘专家专项经费项目

摘　　要：基因型的纯合是保证生物后代性状不发生分离的根本。通常采用连续累代自交同时进行相关性状筛选,或者旁侧PCR筛选等方法纯化转基因生物外源基因。连续累代自交的方法筛选耗时长,工作量大,效率低;旁侧PCR方法技术要求高、专一性强。本研究利用不同斑纹品种混精杂交,结合雄蛾测交,筛选出双亲均为纯合子的蛾圈,作为该品种的纯合品系。该方法简单易行,周期短、效率高,是一种筛选转基因家蚕纯合品系的优良方涛。Homozygosity of genotype is essential for stable inheritance of biological traits in offspring. Conventional methods for purifying exogenous gene in transgenic organism include continuous self-crossing and selection of related traits during each generation,or selection by flanking PCR method. Continuous self-crossing and selection method is time consuming,labor intensive and inefficient,while flanking PCR method needs proficient skills and specific techniques. In this study,we used mixed sperm fertilization of different marking silkworm( Bombyx mori) varieties and test-crossing of male moths to select the offspring whose parents were homozygous. And the selected offspring was taken as the homozygous line. This method is simple and feasible,with short cycle and high efficiency. It is an excellent method for screening homozygous lines of transgenic silkworm.

关 键 词：家蚕 测交 混精杂交 纯合子 转基因阳性率 

分 类 号：S882[农业科学—特种经济动物饲养]