解淀粉芽胞杆菌K103对黄瓜穴盘苗的促生作用  

Growth-Promoting Effects of Bacillus amyloliquefaciens K103 on Cucumber Plug Seedlings

作　　者：董春娟[1] 王玲玲[1] 李亮[1] 秦宇轩[2] 李平兰[2] 尚庆茂[1] DONG Chunjuan;WANG Lingling;LI Liang;QIN Yuxuan;LI Pinglan;and SHANG Qingmao(Key Laboratory of Biological and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]农业部园艺作物生物学与种质创制重点实验室,中国农业科学院蔬菜花卉研究所,北京100081 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《园艺学报》2018年第11期2199-2208,共10页Acta Horticulturae Sinica

基　　金：国家重点研发计划支持项目(2016YED0201003);公益性行业(农业)科研专项经费项目(201303014);国家现代农业产业技术体系建设专项资金项目(CARS-23);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS).

摘　　要：为了探明解淀粉芽胞杆菌(Bacillusamyloliquefaciens)K103对黄瓜穴盘苗的促生效果及作用机理,以草炭、蛭石、珍珠岩混合物料为基质,播种前基质接种K103,测定黄瓜穴盘苗生长发育参数,并采用454焦磷酸测序技术分析穴盘苗根际细菌群落结构和多样性。结果表明:解淀粉芽胞杆菌K103具有较高的固氮和溶解有机磷活性;基质接种K103提高了黄瓜穴盘苗根系活力和矿质养分吸收积累量,促进黄瓜生长;K103可在黄瓜穴盘苗根际定殖,并显著提高了根际食菌蛭弧菌,鞘氨醇单胞菌CHNTR37、CL01,德氏嗜酸菌,鹰嘴豆中慢生根瘤菌等9种细菌的相对丰度,这些细菌对植物多具有促生潜力。:To investigate the growth-promoting effects and mechanisms of Bacillus amyloliquefaciens K103 on cucumber plug seedlings,K103 was inoculated into the substrate before sowing,and the growth parameters,mineral elements aborptions were measured. The rhizosphere bacterial diversities were also detected by 454 pyro sequencing. The results showed that K103 showed high nitrogen fixation and phosphorus solubilization capacity. K103 inoculation in substrate could promote the growth and development of cucumber plug seedlings by increasing the mineral absorption and root vigor. The 454 sequencing data suggested that K103 could colonize in the rhizosphere,and increase the relative abundances of nine bacteria species,including Bdellovibrio bacteriovorus,Sphingomonas sp. CHNTR37 and CL01,Acidovorax delafieldii,Mesorhizobium ciceri,etc. Most of these species were plant growth- promoting bacteria.

关 键 词：黄瓜 解淀粉芽胞杆菌K103 穴盘苗 促生 根际 

分 类 号：S642.2[农业科学—蔬菜学]