不同浓度舒芬太尼对SGC-7901胃癌细胞活力的影响  

THE IMPACT ON CELL VIABILITY OF SGC-7901 GASTRIC CANCER CELL UNDER DIFFERENT CONCENTRATION OF SUFENTANIL

作　　者：蒋留琴 曹金凤 Jiang Liuqin;Cao Jinfeng(Department of Anesthesiology,215Hospital of Nuclear Industry of Shaanxi Province,Xianyang 712000,China)

机构地区：[1]陕西省核工业二一五医院麻醉科

出　　处：《中国煤炭工业医学杂志》2018年第6期592-595,共4页Chinese Journal of Coal Industry Medicine

基　　金：陕西省科技攻关资助项目(编号:2012K19-09-13)

摘　　要：目的探究不同浓度舒芬太尼对人胃癌SGC-7901细胞活力的影响,以期为临床作出贡献。方法将培养至对数生长期的胃癌SGC-7901细胞采用随机数字表法随机分为对照组(C组)、舒芬太尼O.5ng/m1组(F1组)、5ng/ml组(F2组)、50ng/ml组(F3组)和500ng/ml组(F4组),分别以0,0.5,5,50,500ng/ml浓度的舒芬太尼作用于胃癌细胞,通过FDA/PI荧光双染观察舒芬太尼作用后胃癌SGC-7901细胞存活情况,并于孵育24h、48h和72h后用CCK-8试剂盒检测细胞的活力,于孵育24h后用流式细胞仪和AnnexinV-FITC/PI双标记检测细胞周期分布和细胞凋亡率。结果随着舒芬太尼浓度的增高,胃癌SGC-7901细胞死亡比例不断增加,正常细胞存活比例下降,胃癌SGC-7901细胞活力也下降;F4组细胞12h、24h、48h时和F3组细胞24h、48h时活力下降较C组明显,差异有统计学意义(P<0.05);胃癌SGC-7901细胞凋亡率随着舒芬太尼浓度的增加而升高,其中F2~F4组胃癌SGC-7901细胞凋亡率升高较C组明显,差异有统计学意义(P<0.05);F1组停滞在S期和G2/M期的比例与C组比较,差异无统计学意义(P>0.05);F2~F4组停滞在S期的比例明显下降,而停滞在G2/M期的比例明显升高(P<0.05),且随着舒芬太尼浓度的升高,越来越多的细胞被阻滞于G2/M期。结论舒芬太尼浓度≥5ng/ml时,可剂量依赖性抑制胃癌SGC-7901细胞的活力,其机制可能与诱导细胞凋亡,使细胞停滞于G2/M期有关。Objective To explore the impact on cell viability for SGC-7901 gastric cancer cell under different Sufentanil concentration,in order to contribute to the clinical work.Methods We picked up gastric cancer SGC-7901 cell which in the exponential phase and divided randomly them into 5 groups:control group(group C),Sufentanil 0.5 nmol/L(group F1),Sufentanil 5 nmol/L(group F2),Sufentanil 50 nmol/L(group F3),Sufentanil 500 nmol/L(group F4).FDA/PI staining was used to seperate living gastric cancer SGC-7901 cells from dead cells,and then cultivated them for 6,12,24 and 48 hours,the reproduction ability of these cells was detected by CCK-8 assay,and the cell cycle and apoptosis rate for the cancer cell was tagged by Flow Cytometry and Annexin V-FTC/PI after 24 hours.Results With the increasing of concentrations of Sufentanil,the proportion of gastric cancer SGC-7901 cell death increased,and the proportion of normal cell declined;cell vitality decreased which was observed.Compared with group C,cell vitality in group F4 at 12,24 and 48 h and group F3 at 24 and 48 h were decreased significantly(P<0.05);the apoptosis rate in group F2 to F4 were higher than group C(P<0.05);the apoptotic SGC-7901 cells increased with the concentration of Sufentanil increased.And compared with group C,much more cells were arrested in G2/M phase in group F2 to F4 while lower in S phase(P<0.05).Conclusion When the concentration of Sufentanil is above 5 nmol/L,the drug can dose dependently inhibit the viability of SGC-7901 gastric cancer cell,which mechanism may be associated with the leading of cell apoptosis and stop the cell at its G2/M phase.

关 键 词：舒芬太尼 胃癌细胞 细胞活力 

分 类 号：R735.2[医药卫生—肿瘤]