局部给予5-羟色胺2受体激动剂或拮抗剂对SD大鼠舌下神经核兴奋性的影响  

Effects of local use of 5-hydroxytryptamine-2 receptor agonist and antagonist on excitability of hypoglossal nucleus in SD rats

作　　者：王伟[1] 王广发[2] 闫春良[1] 薛旗山[1] 杨国辉 胡系伟 Wang Ivei;Wang Guangfa;Yan Chunliang;Xue Qishan;Yang Guohui;Hu Xiwei(Department of Respiratory Medicine,Beijing Aerospace General Hospital,Beijing 100076,China;Department of Respiratory Medicine,Peking University First Hospital,Beijing 100034,China;Intensive Care Unit,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;Department of Respiratory Medicine,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]北京航天总医院呼吸内科,100076 [2]北京大学第一医院呼吸内科,100034 [3]贵州医科大学附属医院重症医学科,贵阳550004 [4]贵州医科大学附属医院呼吸内科,贵阳550004

出　　处：《中国医药》2018年第12期1885-1888,共4页China Medicine

基　　金：国家自然科学基金(81241004)~~

摘　　要：目的观察5-羟色胺2(5-HT_2)受体激动剂及5-HT_2受体拮抗剂对SD大鼠舌下神经核兴奋性的影响。方法将16只成年雄性SD大鼠完全随机分为A、B组,各8只,均置入微透析导管于其舌下神经核。A组通过微透析导管向舌下神经核泵入新鲜人工脑脊液(ACSF)、新鲜5-HT_2受体激动剂溶液,B组通过微透析导管向舌下神经核泵入新鲜ACSF、新鲜5-HT_2受体拮抗剂溶液,监测A组未给药时、泵入ACSF时、泵入5-HT_2受体激动剂溶液时和B组未给药时、泵入ACSF时、泵入5-HT_2受体拮抗剂溶液时的颏舌肌肌电,利用区间测量方法计算颏舌肌肌电面积。结果 A组未给药时、泵入ACSF时、泵入5-HT_2受体激动剂时大鼠颏舌肌肌电面积分别为(0. 80±0.11)、(0. 82±0.12)、(0.93±0. 14) mV·s,未给药时与泵入ACSF时肌电面积比较,差异无统计学意义(P=0.280),泵入5-HT_2受体激动剂时肌电面积明显大于未给药时与泵入ACSF时,差异均有统计学意义(P=0. 005、0. 005)。B组未给药时、泵入ACSF时、泵入5-HT_2受体拮抗剂时大鼠颏舌肌肌电面积分别为(1.05±0.18)、(1.04±0.19)、(0.73±0.13)mV·s,未给药时与泵入ACSF时肌电面积比较,差异无统计学意义(P=0. 955),泵入5-HT_2受体拮抗剂时肌电面积明显小于未给药时与泵入ACSF时,差异均有统计学意义(P=0.003、0.003)。结论 5-HT_2受体激动剂可以提高舌下神经核的兴奋性,5-HT_2受体拮抗剂可以降低舌下神经核的兴奋性。Objective To observe the effects of 5-hydroxytryptamine-2(5-HT2)receptor agonist and antagonist on hypoglossal nerve nuclear excitability in SD rats.Methods Sixteen adult male SD rats were randomly divided into group A and group B,with 8rats in each group.The group A was injected fresh artificial cerebrospinal fluid(ACSF)and 5-HT2receptor agonist,and the group B was injected ACSF and 5-HT2receptor antagonist through microdialysis catheter into hypoglossal nucleus.Genioglossus electromyography was monitored before drug use,after giving ACSF and 5-HT2agonist/antagonist.Results In the group A,genioglossus electromyography areas before drug use[(0.80±0.11)mV·s]and after giving ACSF[(0.82±0.12)mV·s]were similar(P =0.280);the electromyography area after giving 5-HT2agonist [(0.93±0.14)mV·s ]was significantly greater than that before drug use and after using ACSF(P =0.005,0.005).In the group B,genioglossus electromyography areas before drug use[(1.05±0.18)mV·s]and after using ACSF[(1.04±0.19)mV·s]were similar(P=0.955);the electromyography area after giving 5-HT2antagonist [(0.73±0.13)mV·s ]was significantly smaller than that before drug use and after giving ACSF(P =0.003,0.003).Conclusion 5-HT2receptor agonist can improve the excitability of hypoglossal nucleus ;5-HT2 receptor antagonist can inhibit the excitability of hypoglossal nucleus.

关 键 词：舌下神经核 SD大鼠 5-羟色胺2受体 

分 类 号：R741[医药卫生—神经病学与精神病学]