特异性沉默hTERT基因表达对卵巢癌细胞增殖、凋亡及p53、p21的表达的影响  

Effect of special inhibition of hTERT gene expression on proliferation and apoptosis of ovarian cancer cells and expressions of p53 and p21

作　　者：徐惠昌 晏红[2] 朱薇[2] 骆婧 赵兵善 XU Hui-Chang;YAN Hong;ZHU Wei(People's Hospital of Minqin County,Wuwei,Gansu 733399,China)

机构地区：[1]甘肃省民勤县人民医院,甘肃武威733399 [2]兰州大学第二医院病理科,甘肃兰州730030 [3]甘肃省民勤县食品药品监督管理局,甘肃武威733399

出　　处：《中国妇幼保健》2018年第23期5583-5586,共4页Maternal and Child Health Care of China

摘　　要：目的探索特异性沉默hTERT基因表达对卵巢癌细胞增殖凋亡及p53和p21表达的影响,为临床诊治提供参考依据。方法采用分子克隆技术构建表达hTERTshRNA的载体,特异性沉默hTERT基因表达;WestenBlot法、MTT比色法、流式细胞仪检测抑制hTERT基因表达对卵巢癌细胞p53表达及其增殖和凋亡的影响。结果pSIREN-hTERTshRNA转染有效阻抑A2780细胞中hTERT蛋白表达,抑制率为77%;随着A2780细胞中hTERT蛋白表达降低,p53和p21表达均明显增加;与空白对照及转染pSIREN-Con对照质粒相比,pSIREN-hTERT转染的A2780细胞生长速度明显降低;pSIREN-hTERT转染48hA2780细胞凋亡率为(26.76±7.42)%,明显高于对照质粒pSIREN-Con转染组(3.73±0.78)%及空白对照组(1.33±0.15)%。结论hTERTshRNA重组质粒可特异性抑制卵巢癌细胞hTERT的表达,诱导细胞增殖抑制和凋亡,且可能与上调p53和p21的表达有关。Objective To explore the effect of special inhibition of hTERT gene expression on proliferation and apoptosis of ovarian cancer cells and expressions of p53 and p21,provide a reference basis for clinical diagnosis and treatment of ovarian cancer.Methods The vector expressing hTERT shRNA was constructed by molecular cloning technique,hTERT gene expression was inhibited specifically.Westen Blot,MTT assay,and flow cytometry were used to detect the effect of special inhibition of hTERT gene expression on proliferation and apoptosis of ovarian cancer cells and expressions of p53 and p21.Results pSIREN-hTERT shRNA transfection effectively inhibited hTERT protein expression in A2780 cells,the inhibition rate was 77%.With the decrease of hTERT protein expression in A2780 cells,the expression levels of p53 and p21 increased significantly.Compared with blank control group and pSIREN-Con transfection group,the growth rate of A2780 cells transfected by pSIREN-hTERT decreased significantly.At 48 hours after pSIREN-hTERT transfection,the apoptotic rate of A2780 cells was ( 26.76±7.42) %,which was significantly higher than those in pSIREN-Con transfection group and blank control group [( 3.73±0.78) % and ( 1.33±0.15) %] .Conclusion hTERT shRNA recombinant plasmid can specially inhibit hTERT expression in ovarian cancer cells,induce apoptosis,which may be correlated with upregulation of p53 and p21 expressions.

关 键 词：HTERT P53 卵巢癌 细胞增殖 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]