茵陈二陈汤对非酒精性脂肪性肝炎模型细胞IRS-1^ser307、PKB^ser473表达的影响  

Yinchen and Erchen decoction have an effect on NASH model cells via the protein expression of IRS-1^(ser307) and PKB^(ser473)

作　　者：谢添弘 李军祥[2] 毛堂友 郭一[1,2] 陈晨[1,2] 石磊[1,2] 贾博宜 韩亚飞[1,2] 谭祥 陈润花 XIE Tian-hong;LI Jun-xiang;MAO Tang-you;GUO Yi;CHEN Chen;SHI Lei;JIA Bo-yi;HAN Ya-fei;TAN Xiang;CHEN Run-hua(Beijing University of Chinese Medicine,Beijing 100029,China;Department of Gastroenterology,Dong-Fang Hospital Affiliated To Beijing University of Chinese Medicine,Beijing,100078,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院消化科,北京100078

出　　处：《中国中西医结合消化杂志》2018年第11期919-923,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学青年基金(No:8150140762)

摘　　要：[目的]探讨茵陈二陈汤对非酒精性脂肪性肝炎(NASH)模型细胞内IRS-1^(ser307)、PKB^(ser473)蛋白表达的影响;[方法]采用游离脂肪酸500umol/L(棕榈酸:油酸摩尔比例为1:2)刺激干预HepG2细胞,干预24h建立细胞模型,采用茵陈二陈汤含药血清干预模型细胞,干预治疗24h;选用罗格列酮、多烯磷脂酰胆碱含药血清作为治疗观察对照;Western Blot检测茵陈二陈汤对模型细胞内IRS-1^(ser307)、PKB^(ser473)蛋白表达及IRS-1^(ser307)/IRS-1、PKB^(ser473)/PKB的影响。与空白组相比,游离脂肪酸刺激24h后,模型细胞内IRS-1^(ser307)蛋白表达及IRS-1^(ser307)/IRS-1水平明显升高,PKB^(ser473)蛋白及PKB^(ser473)/PKB水平显著下降;与模型组相比,茵陈二陈汤干预24h后,模型细胞内IRS-1^(ser307)蛋白表达(P<0.01)及IRS-1^(ser307)/IRS-1水平(P<0.01)显著降低,PKB^(ser473)蛋白(P<0.05)及PKB^(ser473)/PKB水平(P<0.05)明显升高,且差异具有统计学意义。[结论](1)茵陈二陈汤可通过调节IRS-1^(ser307)、PKB^(ser473)蛋白表达,降低肝细胞IRS-1蛋白活化水平,促进PKB磷酸化,从而达到干预治疗作用;(2)茵陈二陈汤对IRS-1^(ser307)、PKB^(ser473)蛋白的影响作用与罗格列酮及多烯磷脂酰胆碱效果相近。Background:we aimed to study whether YCECD had an effect on IRS-1^ser307 and PKB^ser473 proteins levels in NASH model cell.[Methods]The 500 umol/L free fatty acids(palmitic acid and oleic acid with the molar ratio of 1:2)stimulated HepG2 cells for 24 hours to establish NASH model cell.We established NASH model cell with YCECD containing serum for 24 hours,Rosiglitazone and Polyenylphosphatidylcholine containing serum as the positive control.We detected the effect of YCECD containing serum on IRS-1^ser307,PKB^ser473,IRS-1^ser307/IRS-1,and PKB^ser473/PKB levels in NASH model cell using western blot.[Results]Compared with the control group,IRS-1^ser307 protein and IRS-1^ser307/IRS-1 levels were increased in model cells after 24 hours stimulation,at the same time PKB^ser473 and PKB^ser473/PKB levels were decreased.Compared with model group,YCECD significantly inhibited the increasing of IRS-1^ser307 protein and IRS-1^ser307/IRS-1 levels,and reversed dyregulations of PKB^ser473 and PKB^ser473/PKB levels after 24 hours intervention.[Conclusion]1.YCECD adjusted IRS-1^ser307 and PKB^ser473 proteins expression in NASH model cellby decreasing the activation of IRS-1,and increasing the phosphorylation level of PKB,and achieved the curative effect.2.YCECD had a similar effect with Rosiglitazone and Polyenylphosphatidylcholine on IRS-1^ser307 and PKB^ser473 proteins levels.

关 键 词：非酒精性脂肪性肝炎 茵陈二陈汤 IRS一1^ser307 PKB^ser473 

分 类 号：R575.1[医药卫生—消化系统]