脑络欣通降低局灶性脑缺血再灌注大鼠额顶叶皮质TLR4及TRAF6和TNF-α蛋白的表达  

Naoluoxintong reduces protein expression of TLR4,TRAF6 and TNF-α in frontal and parietal cortex of rats with focal cerebral ischemia reperfusion

作　　者：王丽娜[1] 曹健[2] 朱国旗[1] 范婧婧 丁玲 贾学锋 梁梦茹[1] 王键[1] 胡建鹏[1] WANG Lina;CAO Jian;ZHU Guoqi;FAN Jinxing;DING Ling;JIA Xuefeng;HANG Mengru;WANG Jian;HU Jianpeng(Ministry-of-Education Key Laboratory of Xin'an Medicine,Anhui University of Chinese Medicine,Hefei 230038;Editorial Department of Journal of Anhni University of Chinese Medicine,Anhui University of Chinese Medicine,Hefei 230038;Department of Rehabilitation,Anhni NO.2Provincial People's Hospital,Hefei 230011,China)

机构地区：[1]安徽中医药大学新安医学教育部重点实验室,安徽合肥230038 [2]安徽中医药大学学报编辑部,安徽合肥230038 [3]安徽省第二人民医院康复科,安徽合肥230011

出　　处：《细胞与分子免疫学杂志》2018年第8期702-707,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金："十二五"国家科技支撑计划(2012BAI126B01);安徽省高等学校省级自然科学研究重点项目(KJ2016A405);安徽省卫生计生委医学科研项目(2018SEYL014)

摘　　要：目的观察脑络欣通对脑缺血再灌注大鼠额顶叶皮质Toll样受体4(TLR4)、肿瘤坏死因子受体相关因子6(TRAF6)、肿瘤坏死因子α(TNF-α)蛋白表达的影响。方法采用改良线栓法建立大脑中动脉闭塞再灌注(MCAO/R)大鼠右侧脑缺血模型,SD大鼠随机分为假手术组、模型组、通心络组和脑络欣通组,每组30只;再分为3、7、14 d三个亚组,灌胃给药。Western blot法检测缺血侧额顶叶皮质TLR4、TRAF6的蛋白水平,免疫组织化学染色法检测缺血侧额顶叶皮质缺血半暗带TNF-α的表达。结果与假手术组比较,模型组3、7、14 d,TLR4、TRAF6蛋白水平显著升高;与模型组比较,各治疗组3、14 d的TLR4、TRAF6蛋白水平显著降低,脑络欣通组7 d显著降低;与通心络组比较,脑络欣通组3、7 d的TLR4的蛋白水平降低,14 d的TLR4蛋白水平升高。与假手术组比较,模型组3、7 d的TNF-α蛋白表达的阳性面积显著升高;与模型组比较,脑络欣通组和通心络组7、14 d的TNF-α蛋白表达的阳性面积显著降低,脑络欣通组3 d的TNF-α蛋白表达的阳性面积显著降低;与通心络组比较,脑络欣通组各时间点TNF-α蛋白表达的阳性面积均无明显差异。结论脑络欣通通过降低TLR4、TRAF6、TNF-α蛋白表达,减轻炎症反应,减轻脑缺血再灌注损伤。Objective To investigate the effect of Naoluoxintong on the protein expression of Toll like receptor 4( TLR4),tumor necrosis factor receptor associated factor-6( TRAF6) and tumor necrosis factor-α( TNF-α) in the frontal and parietal cortex of the rats with middle cerebral artery occlusion and reperfusion( MCAO/R). Methods The modified rat model of MCAO/R was established by suture method. SD rats were randomly divided into four groups: a sham control group,a model group,a Tongxinluo group,and a Naoluoxintong group,with 30 rats in each group. Western blotting was used to detect the expression of TLR4 and TRAF6 in the ischemic lateral frontal cortex,and the expression of TNF-α was measured by immunohistochemistry. Results Compared with the sham control group,the model group had significantly higher expression of TLR4 and TRAF6 on the 3 rd,7 th and 14 th days,which were reduced by Naoluoxintong and Tongxinluo treatments. Interestingly,the expression of TLR4 on the 3 rd,7 th and 14 th days were lower in the Naoluoxintong group as compared with those in the Tongxinluo group. Immunohistochemistry showed that TNF-α expression was also enhanced in the model group on the 3 rd and 7 th days,which were reduced by Naoluoxintong and Tongxinluo treatments. However,TNF-α expression at the different time points was no significantly difference between the Naoluoxintong and Tongxinluo groups. Conclusion Naoluoxintong could reduce MCAO/R-induced expression of TLR4,TRAF6 and TNF-α,which might be related to the effects of Naoluoxintong in cerebral ischemia injury.

关 键 词：脑缺血再灌注 脑络欣通 Toll样受体4(TLR4) 肿瘤坏死因子受体相关因子6(TRAF6) 肿瘤坏死因子α(TNF-α) 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R364.4.[医药卫生—临床医学]