胆囊癌组织中p57^(KIP2)、cyclin D1和cyclin E的表达及临床意义  

Expressions and clinical significances of p57^(KIP2), cyclin D1 and cyclin E in primary carcinoma of the gallbladder

作　　者：罗祖强[1] 庄志泉 涂晓萌 LUO Zuqiang;ZHUANG Zhiquan;TU Xiaomeng(Department of Pathology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China;Department of Medical Imaging, the First Clinical College, Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China;Central Laboratory, Eye Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China)

机构地区：[1]温州医科大学附属第一医院病理科,浙江温州325000 [2]温州医科大学第一临床医学院医学影像学系,浙江温州325000 [3]温州医科大学附属眼视光医院中心实验室,浙江温州325000

出　　处：《中国癌症杂志》2018年第11期801-806,共6页China Oncology

摘　　要：背景与目的:原发性胆囊癌(primary carcinoma of the gallbladder,PCG)是死亡率极高的恶性肿瘤,其恶变的机制目前尚未明确。前期研究发现,p57^(KIP2)在人类多种恶性肿瘤中异常表达。本研究拟进一步探讨p57^(KIP2)在PCG组织中的表达及临床意义。方法:运用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)、免疫组织化学EliVision法分别检测60例PCG、20例胆囊腺瘤(adenoma of the gallbladder,AG)和20例慢性胆囊炎(chronic cholecystitis,CC)组织中p57^(KIP2)、cyclin D1、cyclin E mRNA表达及蛋白水平。结果:p57^(KIP2) mRNA及蛋白在PCG、AG和CC中的表达逐渐升高,两两比较差异有统计学意义(P<0.05)。Cyclin D1、cyclin E mRNA及蛋白在PCG、AG和CC中的表达逐渐降低,PCG与AG比较、PCG与CC比较,差异有统计学意义(P<0.05)。在PCG组织中,p57^(KIP2)蛋白的表达与临床分期、组织学分级及淋巴结转移有关(P<0.05)。Cyclin D1蛋白的表达与临床分期有关(P<0.05)。p57^(KIP2)与cyclin D1的表达呈负相关(P<0.05),p57^(KIP2)与cyclin E的表达呈负相关(P<0.05),cyclin D1与cyclin E的表达呈正相关(P<0.05)。结论:p57^(KIP2)表达的降低与cyclin D1、cyclin E表达的增加可能是PCG的发生机制之一;检测p57^(KIP2)、cyclin D1及cyclin E对PCG的预后判断有重要意义。Background and purpose: Primary carcinoma of the gallbladder(PCG) is a malignant tumor with extremely high mortality, and the mechanism of its malignant transformation is not yet clear. Previous studies found abnormal expression of p57KIP2 in a variety of human malignant tumors. This study aimed to analyze the expressions of p57KIP2, cyclin D1 and cyclin E in PCG and their clinical significances. Methods: The mRNA expression and protein levels of p57KIP2, cyclin D1 and cyclin E in 60 cases of PCG, 20 cases of adenoma of the gallbladder(AG) and 20 cases of chronic cholecystitis(CC) were detected by real-time fluorescence quantitative polymerase chain reaction(RTFQ-PCR) and immunohistochemistry, respectively. Results: The mRNA expression and protein levels of p57KIP2 in PCG, AG and CC increased gradually, and there were significant differences among the three groups(P<0.05). The mRNA expression and protein levels of cyclin D1 and cyclin E in PCG, AG and CC decreased gradually. The difference between PCG and AG, or between PCG and CC was statistically significant. In PCG tissues, the protein expression of p57KIP2 was associated with clinical stage, histological grade and node metastasis(P<0.05). The protein level of cyclin D1 was associated with clinical stage(P<0.05). There was a negative correlation between the expressions of p57KIP2 and cyclin D1, or p57KIP2 and cyclin E. However, the correlation between cyclin D1 and cyclin E expressions was positive(P<0.05). Conclusion: Reduced level of p57KIP2 and overexpression of cyclin E may be one of the mechanisms underlying carcinogenesis of PCG. p57KIP2, cyclin D1 and cyclin E were independent prognostic factors for PCG.

关 键 词：原发性胆囊癌 P57KIP2 CyclinD1 CYCLINE 实时荧光定量聚合酶链反应 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]