粘虫对高效氯氰菊酯抗性机制的初步研究  

Preliminary research on the resistance mechanism of Mythimna separata to beta-cypermethrin

作　　者：赵玉玉[1] 李伯辽 李梅梅 仵均祥[1] 李怡萍[1] ZHAO Yu-Yu;LI Bo-Liao;LI Mei-Mei;WU Jun-Xiang;LI Yi-Ping(College of Plant Protection,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学植物保护学院,杨凌712100

出　　处：《应用昆虫学报》2018年第5期857-864,共8页Chinese Journal of Applied Entomology

基　　金：公益性行业(农业)科研专项(201403031);国家"十三五"重点研发专项(2017YFD0201807);陕西省农业科技创新转化项目

摘　　要：【目的】通过粘虫高效氯氰菊酯抗性、敏感品系生化及分子机制研究,明确与抗药性产生相关的具体机制。【方法】采用室内生物测定、生化分析和分子技术,研究粘虫抗、感品系增效剂的增效作用、解毒酶活性变化及钠离子通道序列变化。【结果】增效剂胡椒基丁醚(PBO)和磷酸三苯酯(TPP)对高效氯氰菊酯的增效作用明显,抗敏增效比分别为5.50和3.40。粘虫高效氯氰菊酯抗性品系酯酶(EST)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFOs)活性均高于敏感品系,其比活力分别为2.45、1.73和1.70,其中抗、感品系的酯酶和多功能氧化酶比活力差异都达到了显著水平(P<0.05)。通过比较粘虫抗性和敏感品系钠离子通道基因ⅡS4-S6片段,未发现与击倒抗性有关的突变。【结论】酯酶和多功能氧化酶可能在粘虫对高效氯氰菊酯的抗性发展中起着重要的作用。[Objectives]To identify the mechanism responsible for beta-cypermethrin resistance in the oriental armyworm.[Methods]The detoxification enzyme activity and sequence alignment in beta-cypermethrin resistant and susceptible strains of the oriental armyworm were determined by bioassay,biochemical analysis and molecular technology. [Results]The results of synergism tests show that triphenyl phosphate (TPP)and piperonyl buoxide (PBO)have an obvious synergistic effect,resulting in 5.50 and 3.40-fold synergist ratios,respectively.Detoxification enzymes tests show that the activity of esterase (EST),glutathione S-transferase (GSTs)and mixed function oxidase (MFOs)in the resistant strain were 2.45,1.73 and 1.70 fold,respectively,that in the susceptible strain.However,only the difference in EST and MFO activity between strains was statistically significant (P<0.05).No mutations associated with knockdown resistance were identified by comparing the sodium channel gene Ⅱ S4-S6 fragment of resistant and susceptible strains.[Conclusion]EST and MFO may play an important role in the development of beta-cypermethrin resistance in M.separata.

关 键 词：粘虫 高效氯氰菊酯 抗性机制 解毒酶 钠离子通道 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]