低能离子注入介导的异常汉逊酵母菌基因组重复序列的突变研究  

Mutation of Repeat Sequences in Hansenula Anomala Genome Mediated by Low-energy Ion Beam Implantation

作　　者：张寒玉 王婷 唐朝 冯光文[1] 钱卫东 蔡长龙[3] 毛培宏[1] ZHANG Hanyu;WANG Ting;TANG Chao;FENG Guangwen;QIAN Weidong;CAI Changlong;MAO Peihong(Research Center of Ion Beam Bwtechnology,School of Physics Science and Technology,Xinjiang University,Urumqi Xinjiang 830046,China;School of Food and Biological Engineering,Shaanxi Univers of Sczencc &Technology,Xi'an Shaanxi 710021,China;3.Research Center of Ion Beam Biotechnology and Biodiversity,Xi'an Technological University,Xi'an Shaanxi 710032,China)

机构地区：[1]新疆大学物理科学与技术学院,离子束生物技术中心,新疆乌鲁木齐830046 [2]陕西科技大学食品与生物工程学院,陕西西安710032 [3]西安工业大学离子束生物工程与生物多样性研究中心,陕西西安710032

出　　处：《新疆大学学报（自然科学版）》2018年第4期479-486,共8页Journal of Xinjiang University(Natural Science Edition)

基　　金：国家自然科学基金(11575149,31760016);陕西科技大学博士科研项目(126021759)资助

摘　　要：重复序列是真核生物基因组的重要组成部分,对生物的系统发育与进化、基因表达与调控有重要作用.为了进一步认识低能离子注入对酵母菌基因组结构的辐射效应,在全基因组de novo测序的基础上,本研究利用生物信息学方法,对低能离子注入前后异常汉逊酵母菌基因组中重复序列的突变特征进行了研究.在串联重复序列方面,离子注入导致异常汉逊酵母菌基因组中微卫星DNA序列和小卫星DNA序列的总长度分别缺失了147bp和5 487bp;多种类型的SSR的重复基序不仅发生了单碱基和多碱基突变,其数量和拷贝数也发生了不同程度的变化,其中优势三碱基重复基序AAC增加了2条,GAG和ATC分别突变为AGG和ATG,四碱基重复基序的GAAT和GTTG分别突变为GTTA和TTCA,五碱基和六碱基重复基序中分别有9种和28种发生了碱基突变;离子注入使高拷贝区的SSR数目增加了1倍,并导致重复单元为47bp和56bp的小卫星DNA序列缺失,同时新增了重复单元为35bp、58bp和59bp的三种小卫星DNA序列.在散在重复序列方面,离子注入导致LTR缺失5条、DNA转座子缺失19个、RC缺失1个,而LINE新增加12条,新增LINE总长度达1 568bp.研究结果为低能离子注入介导的酵母菌基因组突变与进化提供了分子证据,为异常汉逊酵母菌的分子育种和SSR分子标记的开发提供理论基础.Repeat sequence is an important part of the eukaryotic genome and play an important role in the phylogeny and evolution of organisms, gene expression and regulation. In order to further understand the radiation effect of low energy ion implantation on the genome structure of yeast, on the basis of genomewide de novo sequencing, this study used bioinformatics methods to characterize the mutation of repeat sequences in the genome of Hansenula anomala. The results of tandem repeats showed that ion implantation led to the deletion of 147 bp and 5 487 bp in the total length of microsatellite and minisatellite DNA sequences, respectively. The repeat motifs of multiple types of SSRs not only occurred mononucleotide and polynucleotide mutations, but also varied in the quantity and copy number, in which the dominant trinucleotide repeat motif AAC increased by 2, GAG and ATC were respectively mutated to AGG and ATG,GAAT and GTTG of the tetranucleotide repeat motif were respectively mutated to GTTA and TTCA, 9 and 28 base mutations were found in the pentanucleotide and hexanucleotide repeat motifs, respectively;Ion implantation increased the number of SSRs in the high-copy region by a factor of 1, and resulted in the deletion of 47 bp and 56 bp minisatellite DNA sequences, at the same time, three minisatellite DNA sequences with 35 bp, 58 bp and 59 bp repeat units were added. The results of studies on interspersed repeats showed that ion implantation resulted in 5 LTR deletions, 19 DNA transposon deletions, and 1 RC deletions,while LINEs had a new 12 additions and the new LINE total length was 1 568 bp. The results of this study provide molecular evidence for low-energy ion implantation-mediated yeast genome mutation and evolution,providing a theoretical basis for the molecular breeding of Hansenula anomala and the development of SSR molecular markers.

关 键 词：离子注入 异常汉逊酵母菌 基因组 串联重复序列 散在重复序列 

分 类 号：Q691[生物学—生物物理学]