分级醇沉柳茶多糖的含量与单糖组成及抗氧化性研究  

Study on the content,monosaccharide composition and antioxidant activity of the Sibiraea angustata polysaccharides precipitated by gradient ethanol

作　　者：卫阳飞[1] 宋海[1] 宗盈晓 王清云 岳国仁[1] 李建银[3] WEI Yangfei;SONG Hai;ZONG Yingxiao;WANG Qingyun;YUE Guoren;LI Jianyin(Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Hexi University,Zhangye,Gansu,734000P.R.China;College ofChemistry and Chemical engineering,Hexi University,Zhangye,Gansu,734000P.R.China;Pharmacological College of Lanzhou University ,Lanzhou,Gansu,730000P.R.China)

机构地区：[1]河西学院甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000 [2]河西学院化学化工学院,甘肃张掖734000 [3]兰州大学药学院,甘肃兰州730000

出　　处：《华西药学杂志》2018年第6期599-603,共5页West China Journal of Pharmaceutical Sciences

基　　金：甘肃省高等学校科研项目(2018A-088);甘肃省河西走廊特色资源利用重点实验室面上项目(XZ1805);河西学院青年教师科研基金项目(QN2014-15);国家级大学生创新创业训练计划项目(201510740009)

摘　　要：目的研究分级醇沉的柳茶多糖(SAPs)含量、单糖组成及抗氧化活性。方法采用水提、分级醇沉法制备SAPs,用硫酸-苯酚法测定多糖的含量,1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化HPLC法分析单糖的组成,红外分光光度法研究多糖的构型,DPPH·法评价分级多糖的抗氧化活性。结果分级醇沉SAPs含量的高低顺序为60%>40%>80%>20%;葡萄糖、半乳糖和阿拉伯糖在各级醇沉SAPs三氟乙酸水解产物中的含量均最高,其次是半乳糖醛酸、葡萄糖醛酸、甘露糖和核糖,未检测到鼠李糖和木糖;分级醇沉SAPs的抗氧化性优劣顺序为40%>80%>60%>20%,40%醇沉多糖浓度为0.4mg·mL^-1时对DPPH·清除能力与0.04mg·mL^-1抗坏血酸相当,其半数抑制浓度IC50=0.235mg·mL^-1。结论实验结果为柳茶多糖进一步化学、工艺和药理研究提供了参考。OBJECTIVE To study the content,monosaccharide composition and antioxidant activity of different Sibiraea angustata polysaccharides (SAPs)which were precipitated using gradient concentrations of ethanol.METHODS Different SAPs were extracted by water and precipitated with gradient concentrations of ethanol.The content of polysaccharides was determined by sulfuric acid - phenol method.The composition of monosaccharide was determined by HPLC with PMP derivatization.Infrared spectrophotometry was used to study the configuration of SAPs.The antioxidant activity of SAPs was evaluated by DPPH.method.RESULTS The order of the contents of different SAPs was 60%>40%>80%>20%.The contents of glucose,galactose and arabinose were the highest in the TFA hydrolysis products of different SAPs,followed by galacturonic acid,glucuronic acid,mannose and ribose,but no rhamnose and xylose were detected.The order of antioxidant activity was 40%>80%>60%>20%.The scavenging ability of 0.4mg·mL^-1 SAPs which were precipitated by 40%ethanol to DPPH.was almost the same as that of 0.04mg·mL^-1 Vit C,and its IC50 value was 0.235 mg·mL^-1.CONCLUSION The research provides references for further studies on chemical constituents,techniques and pharmacology of SAPs.

关 键 词：柳茶 多糖 分级醇沉 柱前衍生 高效液相色谱法 单糖 红外光谱 抗氧化性 

分 类 号：R917[医药卫生—药物分析学]