黏蛋白1C亚基对食管癌细胞生物学行为的影响  

Effects of mucin1-C on the biological behaviors of esophageal squamous cell carcinoma cells

作　　者：公孙鑫 辛钟伟 史墨[1] 秦启明 姜斌[1] 宋学敏 刘相燕[1] GONGSUN Xin;XIN Zhongwei;SHI Mo;QIN Qiming;JIANG Bin;SONG Xuemin;LIU Xiangyan(Department of Thoracic Surgery,Shandong Provincial Hospital Affiliated to Shandong University,Jinan 250021,Shandong,China)

机构地区：[1]山东大学附属省立医院胸外科,山东济南250021

出　　处：《山东大学学报（医学版）》2018年第10期86-92,共7页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2014HM013)

摘　　要：目的探讨黏蛋白1 C亚基(MUC1-C)在食管鳞癌中的表达定位及在肿瘤细胞恶性生物学行为中的作用。方法 Western blotting检测MUC1-C在食管鳞癌组织及正常组织细胞核内的表达;运用穿膜肽Go-201抑制MUC1-C入核后,集落实验及CCK-8实验检测食管鳞癌细胞的活性、生长和增殖能力;划痕实验及Transwell小室实验检测食管鳞癌细胞迁移及侵袭能力;流式细胞术检测食管鳞癌细胞凋亡率。结果 MUC1-C在食管鳞癌组织细胞核内的表达高于正常组织,抑制MUC1-C入核后食管鳞癌细胞活性、生长、增殖、迁移、侵袭能力均降低,凋亡率增高。结论 MUC1-C通过入核发挥作用,能诱导和促进食管鳞癌细胞的恶性生长、增殖、迁移、侵袭,抑制肿瘤细胞的凋亡。Objective To explore the expression of mucin1-C( MUC1-C) in esophageal squamous cell carcinoma( ESCC) and its role in the malignant biological behaviors of ESCC cells. Methods The expressions of MUC1-C in the ESCC tissues and normal tissues were detected with Western blotting. After MUC1-C entrance into the nucleus was inhibited with Go-201,the activity,growth and proliferation of ESCC cells were assessed with colony-forming assay and CCK-8 test; the cell migration and invasion abilities were measured with wound healing and Transwell assay,respectively; the apoptosis rate was determined with flow cytometry. Results The expression of MUC1-C in ESCC tissues was higher than that in normal tissues. The activity,growth,proliferation,migration and invasion of ESCC cells decreased while the apoptosis rate increased after MUC1-C entrance into the nucleus was inhibited with Go-201. Conclusion MUC1-C can promote the growth,proliferation,migration and invasion while inhibit the apoptosis of ESCC cells.

关 键 词：食管鳞癌 黏蛋白1c亚基 转移 凋亡 

分 类 号：R574[医药卫生—消化系统]