IQ结构域GTP酶激活蛋白3表达对宫颈癌细胞增殖和转移的影响  

Effect of IQ motif-containing GTPase activating protein 3 on the proliferation and migration of cervical cancer cells

作　　者：王永[1] 姜晨[2] 周士英 杨晓玫[3] WANG Yong;JIANG Chen;ZHOU Shiying;YANG Xiaomei(Department of Obstetrics,Jinan Maternity and Child Care Hospital,Jinan 250002,Shandong,China;Department of Gynaecology,Jinan Maternity and Child Care Hospital,Jinan 250002,Shandong,China;Department of Anesthesiology,Qilu Hospital of Shandong University,Jinan 250012,Shandong,China)

机构地区：[1]济南市妇幼保健院产科,山东济南250002 [2]济南市妇幼保健院妇科,山东济南250002 [3]山东大学齐鲁医院麻醉科,山东济南250012

出　　处：《山东大学学报（医学版）》2018年第10期103-109,共7页Journal of Shandong University:Health Sciences

摘　　要：目的探讨宫颈癌组织及细胞系中IQ结构域GTP酶激活蛋白3(IQGAP3)的表达及其对宫颈癌细胞增殖、凋亡和转移的影响。方法应用免疫组化和定量PCR检测IQGAP3在宫颈癌组织及癌旁组织中的表达,利用siRNA敲低IQGAP3在宫颈癌细胞CaSki和HeLa中的表达,通过MTT法和流式细胞术分别检测IQGAP3表达对宫颈癌细胞增殖和凋亡的影响,通过Transwell检测IQGAP3表达对宫颈癌细胞迁移的影响,从而探讨IQGAP3在宫颈癌细胞的增殖、凋亡及迁移过程中的作用。结果 IQGAP3在宫颈癌组织及细胞中高表达,高表达IQGAP3的患者预后较差(χ~2=6. 15,P=0. 01);敲降IQGAP3在宫颈癌细胞CaSki和HeLa的表达后,宫颈癌细胞的增殖受到抑制,凋亡增加,细胞迁移减少,E-cadherin表达增加,N-cadherin表达下调(P均<0. 001)。结论 IQGAP3在宫颈癌组织中表达上调,在宫颈癌细胞增殖和转移过程中发挥重要作用; IQGAP3通过诱导上皮细胞-间充质转变(EMT),促进宫颈癌细胞的转移。Objective To investigate the expression of IQ motif-containing GTPase activating protein 3( IQGAP3) in the cervical cancer tissues and cell lines,and its subsequent influences on cell proliferation,apoptosis and migration. Methods The expression of IQGAP3 was analyzed by immunohistochemistry and real-time PCR in cervical cancer and adjacent normal tissues. The siRNA targeting IQGAP3 was used to knockdown the expression of IQGAP3. The influences of IQGAP3 silencing on cancer cell proliferation,apoptosis and migration were determined by MTT methods,flow cytometry and transwell migration assay,respectively. Results The expression of IQGAP3 was up-regulated in the cervical cancer tissues and cervical cancer cell lines. The up-regulation of IQGAP3 was associated with the poor prognosis of patients with cervical cancer(χ^2= 6. 15,P = 0. 01). Down-regulation of IQGAP3 decreased proliferation and promoted apoptosis in CaSki and HeLa cells. Moreover,as compared with si Scramble transfected cells,the number of migrating cell was significantly decreased in CaSki and HeLa cells due to si-IQGAP3 transfection. In addition,Western blotting analysis showed that knockdown of IQGAP3 increased the expression of epithelial marker E-cadherin and decreased the expression of mesenchymalmarker N-cadherin( all P < 0. 001). Conclusion IQGAP3 is up-regulated in cervical cancer tissues and cells,and its ver-expression promots cell proliferation and the migration capacity in cervical cancer cells. IQGAP3 plays a key role in cervical cancer progression and could be a potential therapeutic target for cervical cancer.

关 键 词：IQ结构域GTP酶激活蛋白3 宫颈癌 细胞增殖 细胞凋亡 上皮细胞-间充质转变 

分 类 号：R737.33[医药卫生—肿瘤]