纳米ZnO对微藻的毒性效应和生物富集的研究  

Toxicity and Bioaccumulation of Zinc Oxide Nanoparticles by Microalgaes

作　　者：汪苹 孙志强 王宇涛[2] 李韶山[2] WANG Ping;SUN Zhiqiang;WANG Yutao;LI Shaoshan(Best Justical Testing Co.,Ltd.,Guangzhou 511400,China;School of Life Science,South China Normal University,Guangzhou 511400,China)

机构地区：[1]广州京诚检测技术有限公司,广东广州511400 [2]华南师范大学生命科学学院,广东广州511400

出　　处：《环境科学与技术》2018年第12期13-19,共7页Environmental Science & Technology

基　　金：2017年广东省自然科学基金博士启动项目资助(2017A030310168)

摘　　要：文章研究了纳米ZnO对小球藻和羊角月牙藻的毒性效应和生物富集与转化效率。纳米ZnO对小球藻和羊角月牙藻细胞的96 h-EC_(50)值分别为17.11和2.26 mg/L。彗星电泳图中拖尾的DNA反映出纳米ZnO诱导了小球藻和羊角月牙藻细胞的DNA损伤,产生了基因毒性。小球藻和羊角月牙藻对培养基中总锌的去除率随时间的延长而增加,在处理96 h后其去除效率可分别达到60.8%和24.1%。双因素方差分析结果表明(P<0.001),小球藻对纳米ZnO具有更强的富集效率。The toxicity, removal and bioaccumulation of zinc oxide nanoparticles(nano-ZnO) by two unicellular green microalgal species, Chlorella vulgaris and Selenastrum capricornutum, were studied. The cytotoxicity of nano-ZnO was concentration-, species-and time-dependent. The 96 h-EC50 values of nano-ZnO to C. vulgaris and S. capricornutum were17.11 and 2.26 mg/L, respectively, suggesting that C. vulgaris was much more tolerant to nano-ZnO than S. capricornutum.The unclear tail in comet assay images reflected that nano-ZnO induced DNA damage in both microalgae. Two-way ANOVA results showed that C. vulgaris had higher removal efficiencies and uptake ability of nano-ZnO than S. capricornutum(P<0.001). Further research is needed to understand the toxicity mechanism of nano-ZnO and the trophic transfer effect of nano-ZnO through the food chain.

关 键 词：纳米氧化物 藻类 生物毒性 生物富集 生物转化 

分 类 号：X172[环境科学与工程—环境科学]