PCR法检测肉制品中鹅源性成分  

Detection of goose-derived ingredients in meat products by PCR

作　　者：李宏铎 夏天 孙晶莹[2] 李亚楠 杨晓东 LI Hong-Duo;XIA Tian;SUN Jing-Ying;LI Ya-Nan;YANG Xiao-Dong(Xi'an Food and Drug Inspection Institute, Xi'an 710068, China;Shaanxi Provincial People's Hospital Central Laboratory, Xi'an 710068, China)

机构地区：[1]西安市食品药品检验所,西安710068 [2]陕西省人民医院中心实验室,西安710068

出　　处：《食品安全质量检测学报》2018年第23期6258-6261,共4页Journal of Food Safety and Quality

基　　金：陕西省科技计划项目(2017ZDXM-NY-052)~~

摘　　要：目的建立一种PCR法检测肉制品中鹅源性成分的方法。方法根据鹅的线粒体DNA(mtDNA)序列设计引物,以9种动物的DNA为模板,利用新设计的鹅的特异性引物进行PCR反应,并用琼脂糖凝胶电泳检测引物的特异性和敏感性。结果通过PCR反应,仅鹅的DNA扩增得到了194bp的目的片段,其余物种DNA和空白对照均无目的片段。扩增产物的核苷酸序列与GENBANK中检索到的相应序列基本相符合。结论该方法特异性强、灵敏度高,适合检测肉制品中的鹅源性成分。Objective To establish a method for determination of goose-derived ingredients in meat products by PCR. Method Primers were designed based on the mitochondrial DNA (mtDNA) sequence of the goose. The DNA of the 9 kinds of animals was used as a template, and the specific primers of the newly designed goose were used for PCR reaction, and the specificity and sensitivity of the primers were detected by agarose gel electrophoresis. Results Through the PCR reaction, only 194 bp of the target fragment was obtained by DNA amplification of the goose, and the remaining DNA and blank control had no target fragment. The nucleotide sequence of the amplified product was substantially identical to the corresponding sequence retrieved in GENBANK. Conclusion The method is highly specific and sensitive, which is suitable for detecting goose-derived ingredients in meat products.

关 键 词：鹅 线粒体DNA 动物源性成分 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]