L-左旋肌肽对人口腔鳞癌干细胞增殖及凋亡作用的实验研究  

Study of the effect of L-carnosine on the proliferation and apoptosis of stem cells in oral squamous cell carcinoma

作　　者：范龙坤[1] 华泽权[2] 马超[1] FAN Long-kun;HUA Ze-quan;MA Chao(Cangzhou Central Hospital,Cangzhou 061001,China)

机构地区：[1]沧州市中心医院,河北沧州061001 [2]沈阳军区总医院口腔科,辽宁沈阳110016

出　　处：《中国美容整形外科杂志》2018年第12期743-746,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的研究L-左旋肌肽对人口腔癌细胞Tca8113的凋亡作用及机制。方法体外培养Tca8113细胞,将对数生长期的Tca8113细胞分为L-左旋肌肽(5、20、50 mmol/L)处理组和空白对照组。按照分组条件分别用L-左旋肌肽5、20、50 mmol/L和生理盐水处理各组对应细胞。采用MTT法检测处理24 h和48 h后各组Tca8113细胞增殖情况;干预48 h后流式细胞仪检测各组Tca8113细胞凋亡和细胞周期情况;Western blot方法检测各处理组48 h后AKT、p-AKT和p38、p-p38蛋白的表达情况。结果 MTT和流式结果显示,L-左旋肌肽对Tca8113细胞的抑制作用依赖于浓度和时间。Western blot结果显示,L-左旋肌肽干预细胞48 h后,随着L-左旋肌肽浓度的提高,p-AKT和p-p38的蛋白表达量降低。结论 L-左旋肌肽通过抑制AKT和MAPK信号通路,抑制Tca8113细胞的增殖,并促进其凋亡。Objective To study the effect of L-carnosine on the proliferation and apoptosis of human oral squamous cell carcinoma cell line Tca8113 and its mechanism. Methods Human oral squamous cell carcinoma cell line Tca8113 were cultured in vitro. Then the cells were divided in the logarithmic growth phase into an L-carnosine(5 mmol/L, 20 mmol/L and 50 mmol/L) and a normal saline treatment group. The proliferation of Tca8113 was measured by MTT assay at 24 and 48 hours. The apoptosis and cell cycle were detected by flowcytometry after 48 hours. The expression of AKT, p-AKT, p38 and p-p38 in each group at 48 hours was detected through Western blot. Results MTT assay and flowcytometry showed that the inhibitory effect of l-carnosine on Tca8113 depends on treatment concentration and time. Western blot results revealed that the expression of p-AKT and p-p38 were down-regulated in an inverse dose-dependent manner after L-carnosine treatment. Conclusion L-carnosine can inhibit the proliferation and promote apoptosis of human oral squamous cell carcinoma cell line Tca8113 through inhibiting the AKT and MAPK signaling pathways.

关 键 词：L-左旋肌肽 人口腔癌细胞 凋亡 增殖 AKT和MAPK 

分 类 号：R739.8[医药卫生—肿瘤]