非小细胞肺癌患者外周血miR-146a水平检测及临床意义  

Detection of miR-146a in peripheral blood in non-small cell lung cancer and its clinical significance

作　　者：李存龙 刘峰 周锋 薛亚妮[4] LI Cun-long;LIU Feng;ZHOU Feng;XUE Ya-ni(Department of Physical Examination,Yan'an University Affiliated Hospital Yan'an 716000,China;Department of Ultrasound,Shangnan County Hospital,Shangluo 726300,China;Clinical Laboratory,Shangnan County Hospital,Shangluo 726300,China;Department of Respiratory,Yan'an University Affiliated Hospital,Yan'an 716000,China)

机构地区：[1]陕西省延安大学附属医院体检科,延安716000 [2]陕西省商洛市商南县医院超声科,商洛726300 [3]陕西省商洛市商南县医院检验科,商洛726300 [4]延安大学附属医院呼吸科,延安716000

出　　处：《中国肿瘤临床与康复》2018年第12期1413-1416,共4页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：陕西省卫生计生科研基金项目(2016D082)

摘　　要：目的探讨非小细胞肺癌(NSCLC)患者外周血miR-146a水平检测及其与肿瘤恶性程度的相关性。方法选取2015年1月至2017年10月间延安大学附属医院收治的接受根治性手术的80例NSCLC患者作为NSCLC组,同期选取确诊为良性病变且进行手术治疗的90例肺部小结节患者作为对照组。比较两组患者术前外周血中miR-146a表达水平、免疫逃逸指标水平以及术中所得肺部病灶中原癌基因表达量的差异,分析NSCLC患者外周血miR-146a水平与肿瘤恶性程度的相关关系。结果 NSCLC组患者外周血中miR-146a表达水平高于对照组患者;外周血中免疫逃逸指标CD4+CD25+调节性T细胞和CD8+CD25+抑制性T细胞的分布比例均高于对照组患者,自然杀伤细胞(NK)的分布比例低于对照组患者;病灶组织中原癌基因Wip1、Pim-1、pokemon和C-Myc mRNA的表达量均高于对照组患者,差异均有统计学意义(均P <0. 05)。NSCLC患者外周血miR-146a水平与机体免疫逃逸程度及原癌基因表达量均直接相关,差异均有统计学意义(均P <0. 05)。结论NSCLC患者外周血miR-146a异常高表达,与肿瘤细胞的免疫逃逸及原癌基因表达量均直接相关,是客观反映肿瘤恶性程度的可靠指标。Objective To detect level of microRNA-146 a in peripheral blood in non-small cell lung cancer( NSCLC) and its correlation with grading of malignancy. Methods Eighty patients with NSCLC who underwent surgical treatment between January 2015 and October 2017 at Yan'an University Affiliated Hospital were selected as NSCLC group,and 90 patients with benign lung lesions undergoing operation at the same time were selected as the control group. The expression of microRNA-146 a in peripheral blood before operation,levels of immune escape indexes in peripheral blood and expression of proto-oncogene in lung lesions were compared between the two groups. The correlation between microRNA-146 a level in peripheral blood and grading of malignancy was analyzed. Results Expression level of microRNA-146 a was higherin NSCLC group than in the control group. Distribution ratio of CD4^+CD25^+regulatory T cells and CD8+CD25+inhibitory T cells were higher in NSCLC group than in the control group. Distribution ratio of natural killer( NK) cells was lower in NSCLC group than in the control group. The expression of proto-oncogenes such as Wip1、Pim-1、pokemon、C-Myc mRNA was higher in NSCLC group than in the control group( all P<0. 05). Level of microRNA146 a in peripheral blood were directly correlated with the degree of immune escape and expression of proto-oncogene in NSCLC patients( all P<0. 05). Conclusion Abnormal high expression of microRNA-146 a is directly related to immune escape of tumor cells and the expression of protooncogenes in NSCLC patients,and it is a reliable index to objectively reflect the degree of malignant tumors.

关 键 词：癌 非小细胞肺 MIR-146A 免疫逃逸 原癌基因 

分 类 号：R734.2[医药卫生—肿瘤]