寄主诱导的基因沉默在丝状真菌中应用的最新研究进展  

Latest Research Progress on the Application of Host Induced Gene Silencing in Filamentous Fungi

作　　者：赵美[1] 皮磊[1] 郑文博 刘艳潇 周而勋[1] 舒灿伟[1] Zhao Mei;Pi Lei;Zheng Wenbo;Liu Yanxiao;Zhou Erxun;Shu Canwei(Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Research Center for Population Microbiology, College of Agriculture, South China Agricultural University, Guangzhou,510642)

机构地区：[1]华南农业大学农学院华南农业大学群体微生物研究中心广东省微生物信号与作物病害防控重点实验室

出　　处：《分子植物育种》2018年第23期7689-7694,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31801677);广东省自然科学基金(博士启动)项目(2016A030310454);国家重点研发计划项目(2017YFD0200900);国家重点研发计划项目(2017YFD0201100)共同资助

摘　　要：寄主诱导基因沉默(host induced gene silencing,HIGS)技术是基于RNAi而发展起来的新技术,它既可以从反向遗传学的方向鉴定植物病原真菌的基因功能,又可以通过在寄主植物中表达沉默病原物特定基因的HIGS载体,以达到控制病原菌扩展,提高作物抗病性的目的。因此HIGS在研究植物病原真菌的致病机理和作物的抗病育种中极具应用前景。HIGS技术从报道以来在植物保护领域得到了广泛的运用,其适用范围广,可以在多种病原真菌中进行应用,特别对于难培养和难以进行遗传转化的真菌。并可以在植物抗病育种中发挥更大空间,为病害的防治提供可靠的策略。本综述总结概括了HIGS技术的原理和它在植物病原丝状真菌研究的最新进展,为该技术在更多植物病原真菌的使用提供参考依据。Host induced gene silencing (HIGS) is a new technology which is developed based on RNA interference. It can not only identify the gene function of plant pathogenic fungi in the direction of reverse genetics, but also express the HIGS vector of the specific gene of silencing disease in the host plant, in order to control the spread of pathogenic bacteria and improve the disease resistance of crops. Therefore, HIGS can be further used to study the pathogenic mechanism of plant pathogenic and crop breeding for disease resistant. HIGS has been commonly used in plant protection since it is developed. It has a wide range of application and can be applied in a variety of pathogenic fungi, especially for the fungi which are difficult to cultivate and difficult to do genetic transformation It is expected to play a greater function in plant resistance breeding and provide a reliable strategy for disease prevention and control. This research reviewed the principle of HIGS and the latest research progress on HIGS in filamentous fungi of plant pathogens, which would provide references for the application of HIGS in more plant pathogens.

关 键 词：寄主诱导基因沉默 丝状真菌 致病基因 抗病育种 

分 类 号：Q78[生物学—分子生物学] S432.44[农业科学—植物病理学]