OsPDR在酵母中异源表达增强了酵母对钴的耐受性（英文）  

Heterologous Expression of OsPDR Enhances The Tolerance to Cobalt in Yeast

作　　者：田思琪 乔坤 王凡红 梁爽 王红 柴团耀 TIAN Si-Qi;QIAO Kun;WANG Fan-Hong;LIANG Shuang;WANG Hong;CHAI Tuan-Yao(College of Life Science, University of The Chinese Academy of Sciences, Beijing 101408, China;Southeast Asia Biodiversity Research Institute, Chinese Academy of Science, Yezin, Nay Pyi Taw 05282, Myanmar)

机构地区：[1]中国科学院大学生命科学学院,北京101408 [2]中国科学院东南亚生物多样性研究中心,缅甸耶津内比都05282

出　　处：《生物化学与生物物理进展》2018年第12期1259-1267,共9页Progress In Biochemistry and Biophysics

基　　金：supported by grants from The National Natural Science Foundation of China(U1632111,61672489);the Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Y4ZK111B01);the Chinese Academy of Sciences(KJRH2015-001)~~

摘　　要：在通过RNA-Seq技术得到的镉响应转录组图谱中,用50μmol/L Cd处理24 h后,一个镉响应金属离子转运蛋白OsPDR被鉴定出其在水稻(Oryza sativa ssp. japonica cv. Nipponbare)茎中的表达量显著上调.本研究中,从水稻(Oryza sativa cv.Nipponbare)中分离了OsPDR基因,并对其金属离子转移活性进行了分析.金属耐受性实验结果表明,过表达OsPDR能提高酵母对Co的耐受性,但对Zn、Ni和Cd的耐受性不强,并且经电感耦合等离子体质谱法(ICP-MS)测定Co含量后,与空载体转化酵母相比,过表达OsPDR的酵母中Co的积累更高.利用共聚焦显微镜观察发现,EGFP-OsP DR融合蛋白定位于液泡膜上.这些数据表明OsP DR可能在Co稳态中起着重要作用.OsPDR在植物中的作用,还需要进一步的研究.A cadmium-responsive metal ion transporter OsPDR,which was highly upregulated in the shoot of rice(Oryza sativa ssp.japonica cv.Nipponbare)at 24 h after treatment with 50 μmol/L Cd,was identified in cadmium-responsive transcriptome profiles analyzed by RNA-Seq.In this study,we isolated OsPDR from rice(Oryza sativa cv.Nipponbare)and the metal ions transport activity of OsPDR was analyzed.The results of metal tolerance experiments showed that overexpression of OsPDR can enhance yeast’s tolerance to Co,but not to Zn,Ni or Cd,and higher accumulation of Co was observed compared to the empty vector-transformed yeast as determined by inductively coupled plasma mass spectrometry(ICP-MS).The EGFP-OsPDR fusion protein was localized to the vacuolar membrane using confocal microscopy.These data suggested that OsPDR might play an important role in Co homeostasis.Additional studies are needed to understand the function of OsPDR in plants.

关 键 词：OsPDR 钴 转运 酵母 

分 类 号：Q5[生物学—生物化学] Q7