放射抗拒性肿瘤细胞株构建研究进展  

Advance of establishing radioresistant tumor cell lines

作　　者：吴波 王勇 朱杰 应申鹏 吕佳铭 熊华才 胡洁 胡炜 Wu Bo;Wang Yong;Zhu Jie;Ying Shenpeng;Lyu Jiaming;Xiong Huaeai;Hu Jie;Hu Wei(Department of Radiation Oncology,Taizhou Central Hospital,Affiliated Hospital of Taizhou University,Taizhou 318000,China;Clinical Laboratory,Taizhou Central Hospital,Affiliated Hospital of Taizhou University,Taizhou 318000,China;Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]台州市中心医院台州学院附属医院放疗科,318000 [2]台州市中心医院台州学院附属医院检验科,318000 [3]温州医科大学,325000

出　　处：《中华放射医学与防护杂志》2018年第12期956-960,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：浙江省公益技术应用研究计划项目(2015C33257).

摘　　要：约70%的恶性肿瘤患者需要放射治疗,局部复发和转移是治疗失败的主要模式,放射抗拒性是重要的因素之一。明确潜在的放射抗拒相关机制至关重要,通过放射线构建放射抗拒性肿瘤细胞株是关键的工作基础。根据分割剂量、照射次数及不同分割剂量组合方式等因素的不同,归纳出4种典型的照射模式:常规照射模式、反复照射模式、梯度照射模式和其他照射模式。不同的照射模式在总照射剂量、构建周期上不同,肿瘤细胞的生物学特性也存在差异。梯度照射模式随着细胞株放射抗拒性增强而逐步提高分割剂量,较好地平衡了分割剂量和照射后细胞恢复至指数期时间,优于其他3种照射模式。临床相关放射抗拒性细胞株具备放射抗拒性表型的同时,保持与亲本细胞株相同的基因型,是未来肿瘤放射抗拒性研究的重要方向。Radiotherapy is necessary for 70% of malignant tumor patients. Local recurrence and metastasis are primary failure models, where radioresistance is one of important factors. It is critical to establish radioresistant tumor cell lines for understanding the mechanism of radioresistance. According to single radiation, fractioned radiation and the compound radiation method, four representative radiation models are classified:conventional radiation, repeated radiation, gradient radiation and other radiation. These different radiation models have difference in total dose and radiation model as well as the biological characteristics. Superior to other three models, the gradient fractioned irradiation model increase fractioned doses gradually along with the enhancement of radioresistance, which favorably balances the fractioned doses and the time of irradiated cells approaching to exponential growth phase. Clinically relevant radioresistant cell line (CRR) with a genotype in consistent with its parental cells is an important research direction on tumor radioresistance.

关 键 词：放射治疗 放射敏感性 放射抗拒性 梯度照射 

分 类 号：R730.55[医药卫生—肿瘤]