丹参酮ⅡA通过调控上皮间质转化抑制食管癌EC9706和KYSE70细胞的凋亡、侵袭和迁移  

Tanshinone ⅡA inhibits apoptosis, invasion and migration of esophageal cancer EC9706 and KYSE70 cells by regulating epithelial-mesenchymal transition

作　　者：周鹏飞 李娟 ZHOU Pengfei;LI Juan(Department of Thoracic Oncology, Cancer Hospital of Meishan, Meishan 620010, Sichuan, China;Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Chengdu Medical College, Chengdu 610500, Sichuan, China)

机构地区：[1]眉山肿瘤医院胸部肿瘤科,四川眉山620010 [2]成都医学院基础医学院病理学与病理生理学教研室,四川成都610500

出　　处：《中国肿瘤生物治疗杂志》2018年第12期1244-1250,共7页Chinese Journal of Cancer Biotherapy

基　　金：四川省教育厅科研项目(No.18ZB0176)~~

摘　　要：目的:探讨丹参酮ⅡA对食管癌EC9706和KYSE70细胞侵袭和迁移的影响及其调控机制。方法:食管癌细胞系EC9706和KYSE70培养完成后分为4组:对照组(加DMSO)和2、4、6μg/ml丹参酮组。采用CCK-8法检测EC9706和KYSE70细胞增殖活力,流式细胞术检测细胞凋亡率,Transwell实验检测细胞侵袭能力,划痕愈合实验检测细胞迁移能力,qRT-PCR和Western blotting实验检测EMT相关蛋白E-cadherin、Snail-2、Vimentin和N-cadherin mRNA和蛋白表达水平。结果:小于6μg/ml的丹参酮ⅡA不影响食管癌EC9706和KYSE70细胞增殖活力;4、6μg/ml丹参酮ⅡA组细胞凋亡率明显高于对照组(均P<0.01);2、4、6μg/ml丹参酮组每个视野下的侵袭细胞数及划痕愈合率明显低于对照组(均P<0.01),且EC9706和KYSE70细胞形态由纺锤状的间充质形态转变为上皮形态。与对照组相比,2、4、6μg/ml丹参酮组E-cadherin表达明显升高,Snail-2、Vimentin和N-cadherin表达明显下降(均P<0.01)。结论:丹参酮ⅡA通过抑制EMT促进食管癌EC9706和KYSE70细胞凋亡,并减弱其侵袭和迁移能力。Objective: To investigate the effect of tanshinone ⅡA on the invasion and migration of esophageal cancer EC9706 and KYSE70 cells, and to explore the underlying mechanism. Methods: Esophageal cancer cells(EC9706 and KYSE70) were divided into4 groups: control group, tanshinone ⅡA groups(2, 4, 6 μg/ml). Cell prliferation viability was measured by CCK-8; Apoptosis was detected by flow cytometry; Invasion was tested by Transwell assay; And migration was measured by Scratch assay. The mRNA and protein levels of E-cadherin, Snail-2, Vimentin and N-cadherin were tested by quantitative Real-time reverse transcription PCR(qRT-PCR)and Western blotting, respectively. Results: Tanshinone ⅡA at concentrations less than 6 μg/ml did not affect the cell viability of esophageal cancer EC9706 and KYSE70 cells. The apoptosis in tanshinone ⅡA(4, 6 μg/ml) groups was significantly higher than that in control group(P< 0.01). The number of invasive cells per field and wound-healing rate in tanshinone ⅡA(2, 4, 6 μg/ml) groups were significantly lower than those in control group(all P<0.01). Moreover, the cell morphology was transformed from a spindle-shaped mesenchymal form into epithelial morphology after tanshinone ⅡA treatment. Compared with control group, the expression of E-cadherin in tanshinone ⅡA groups(2, 4, 6 μg/ml) was significantly up-regulated while the expressions of Snail-2, Vimentin and N-cadherin were significantly down-regulated(all P<0.01). Conclusion: Tanshinone ⅡA promotes apoptosis and attenuates the invasion and migration of esophageal cancer cells by inhibiting the epithelial-mesenchymal transition.

关 键 词：丹参酮ⅡA 食管癌 EC9706细胞 KYSE70细胞 上皮间质转化 凋亡 侵袭 迁移 

分 类 号：R735.1[医药卫生—肿瘤] R7392.11[医药卫生—临床医学]