高效液相色谱-蒸发光散射检测法同时测定牛舌草提取液中的5种单糖  

Simultaneous determination of 5 kinds of monosaccharides in Borago officinalis L. extract by high performance liquid chromatography-evaporative light scattering detector

作　　者：依明.尕哈甫 王晓梅[1] 热娜.卡斯木[1] 胡君萍[1] YIMING-Ga-Ha-Fu;WANG Xiao-Mei;RENA.Ka-Si-Mu;HU Jun-Ping(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011

出　　处：《食品安全质量检测学报》2018年第24期6455-6459,共5页Journal of Food Safety and Quality

基　　金：国家"十二五"科技支撑计划(2012BAI30B02);新疆维吾尔自治区教育厅高校科研计划(XJEDU2016S059)~~

摘　　要：目的建立同时测定牛舌草中5种水溶性糖(鼠李糖、阿拉伯糖、果糖、葡萄糖、半乳糖)的液相色谱-蒸収光散射分析方法。方法各单糖组分经GRACE Prevail ES糖分析柱分离幵与蒸収光散射检测器(evaporative light scattering detector, ELSD)联用,采用V(乙腈):V(水)=72:28作为流动相,流速为1.0 mL/min,蒸収光散射检测器漂移管温度为80℃,空气作为载气,流速为2.0 L/min。结果各单糖的峰面积标准曲线在0.2000～6.400μg范围内均具有良好的线性关系,相关系数在0.999以上,加样回收率在97.94%～103.3%之间;相对标准偏差在1.15%～3.08%之间;所建立的方法对5种糖的检测限分别为60.00、100.0、100.0、60.00、80.00 ng。结论本方法灵敏、准确,可用于牛舌草中水溶性糖含量的质量控制与分析。Objective To establish a method for simultaneous determination of 5 kinds of monosaccharides (rhamnose,fructose,arabinose,glucose and galactose)in Borago officinalis L.extract by high performance liquid chromatography with evaporative light scattering detector (ELSD).Methods The monosaccharides samples were separated by GRACE Prevail carbohydrate ES column using acetonitrile-water (72:28,V:V)as the mobile phase at a flow rate of 1.0mL/min.Drift tube temperature was 80℃ and air carrier gas flow rate was 2.0L/min.Results The peak area of each monosaccharide had good linear relationships in the range of 0.2000-6.4000μg,with the correlation coefficients all above 0.999.The recoveries were ranging from 97.94% to 103.3%,with the relative standard deviations of 1.15%-3.08%.The limits of detection for the 5 kinds of monosaccharides were 60.00,100.0,100.0, 60.00 and 80.00 ng,respectively.Conclusion The method is sensitive and accurate,which can be used for the quality control and analysis of water-soluble monosaccharides in Borago officinalis L.

关 键 词：高效液相色谱法 蒸发光散射检测器 牛舌草 单糖 

分 类 号：R284[医药卫生—中药学]