生长素及其类似物增强香菇耐高温性的研究  

Auxin and auxin analogues enhancing the thermotolerance of Lentinula edodes

作　　者：周莎莎 王刚正 罗义 马超君[1] 龚钰华[1] 边银丙[1] 周雁[1] ZHOU Sha‐Sha;WANG Gang‐Zheng;LUO Yi;MA Chao‐Jun;GONG Yu‐Hua;BIAN Yin‐Bing;ZHOU Yan(Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China)

机构地区：[1]华中农业大学植物科学技术学院应用真菌研究所,湖北武汉430070

出　　处：《菌物学报》2018年第12期1723-1730,共8页Mycosystema

基　　金：国家自然科学基金(34116162);湖北省技术创新专项重大项目(2016ABA100)~~

摘　　要：高温胁迫影响香菇的品质和产量。以香菇Lentinula edodes热敏感菌株YS3357为试验材料,研究了外源生长素及其类似物对香菇菌丝体高温胁迫下氧化损伤的缓解效应。结果表明,外源添加IAA、NAA和2,4-D能够显著提高热敏感菌株YS3357的耐热能力。外源生长素类物质在一定程度上可以抑制超氧阴离子(O2-)产生,降低脂氧合酶(LOX)活性和硫代巴比妥酸反应物(TBARS)含量,提高超氧化物歧化酶(SOD)活性,可能与缓解香菇菌丝体由于高温胁迫所引起的氧化损伤有关。本研究重点探讨外源添加生长素及其类似物对热胁迫下香菇热敏感菌株YS3357菌丝生长、生理特性及抗氧化胁迫能力的影响,为进一步阐明食用菌抗高温胁迫机制奠定了一定的理论基础。Heat stress affects the quality and production of Lentinula edodes. In this study, using sensitive-heat strain YS3357 of L.edodes as tested strain, the alleviative effects of exogenous auxin and auxin analogues on heat-induced oxidative damage inLentinula edodes under heat stress were investigated. The results indicated that exogenous IAA, NAA and 2,4-D improveddramatically the thermotolerance of the sensitive-heat strain YS3357. Exogenous auxin and auxin analogues could suppress thegeneration of O2-, decrease the activity of LOX and the content of TBARS, and improve the activity of SOD, which have relationswith alleviating the effect of oxidative damage induced by heat stress on the mycelia of Lentinula edodes. This study mainlyexplored the effects of exogenous auxin and auxin analogues on the mycelial growth, physiological capacity and resistance tooxidative stress for the sensitive-heat strain YS3357 under heat stress, providing the theoretical foundation on furtherillumination of the mechanism of resistance-heat stress for the edible fungi.

关 键 词：香菇 生长素 高温胁迫 活性氧 

分 类 号：S646.12[农业科学—蔬菜学]